Human mast cells take up and hydrolyze anandamide under the control of 5-lipoxygenase and do not express cannabinoid receptors.

@article{Maccarrone2000HumanMC,
  title={Human mast cells take up and hydrolyze anandamide under the control of 5-lipoxygenase and do not express cannabinoid receptors.},
  author={Mauro Maccarrone and Laura Fiorucci and Fulvio Erba and Monica Bari and Alessandro Finazzi-Agr{\`o} and Franca Ascoli},
  journal={FEBS letters},
  year={2000},
  volume={468 2-3},
  pages={176-80}
}
Human mast cells (HMC-1) take up anandamide (arachidonoyl-ethanolamide, AEA) with a saturable process (K(m)=200+/-20 nM, V(max)=25+/-3 pmol min(-1) mg protein(-1)), enhanced two-fold over control by nitric oxide-donors. Internalized AEA was hydrolyzed by a fatty acid amide hydrolase (FAAH), whose activity became measurable only in the presence of 5-lipoxygenase, but not cyclooxygenase, inhibitors. FAAH (K(m)=5.0+/-0.5 microM, V(max)=160+/-15 pmol min(-1) mg protein(-1)) was competitively… CONTINUE READING

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