Human marrow mesenchymal stem cell culture: serum-free medium allows better expansion than classical alpha-MEM medium.

@article{Meuleman2006HumanMM,
  title={Human marrow mesenchymal stem cell culture: serum-free medium allows better expansion than classical alpha-MEM medium.},
  author={Nathalie Meuleman and Tatiana Tondreau and Alain Delforge and Marielle Dejeneffe and Martine Massy and Mark Libertalis and Dominique Bron and Laurence Lagneaux},
  journal={European journal of haematology},
  year={2006},
  volume={76 4},
  pages={309-16}
}
The expansion of mesenchymal stem cells (MSCs) strongly depends on the culture conditions and requires medium supplemented with 10-20% fetal calf serum (FCS) to generate relevant numbers of cells. However, the presence of FCS is a major obstacle for their clinical use. Therefore, we have evaluated the capacity of expansion of MSC in a commercial serum-free medium (UC) supplemented with a serum substitute (ULTROSER) in comparison with a classical medium alpha-MEM containing 15% FBS. Bone marrow… CONTINUE READING

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