Human lymphocyte potassium content during the initiation of phytohemagglutinin‐induced mitogenesis

@article{Segel1976HumanLP,
  title={Human lymphocyte potassium content during the initiation of phytohemagglutinin‐induced mitogenesis},
  author={George B. Segel and Marshall A. Lichtman and Marsha M. Hollander and Bruce R. Gordon and Martin R. Klemperer},
  journal={Journal of Cellular Physiology},
  year={1976},
  volume={88}
}
The K+ content of human lymphocytes has been examined during the initial 24 hours after exposure of cells to phytohemagglutinin (PHA). We have reconfirmed that lymphocyte K+ exchanges rapidly for extracellular counterions during preparative washing if cells are exposed to PHA. By using a technique to measure cation content which does not require removal of cells from their culture medium, we have shown that K+ does not change for 24 hours following PHA treatment. Previous reports have… 
TRANSMEMBRANE K+ TURNOVER BY PHYTOHEMAGGLUTININ (PHA) – STIMULATED HUMAN LYMPHOCYTES
Publisher Summary This chapter focuses on transmembrane K+ turnover by phytohemagglutinin (PHA)-stimulated human lymphocytes. The alteration of the plasma membrane may be critical in the occurrence
Potasssium transport in human blood lymphocytes treated with phytohemagglutinin.
TLDR
It is confirmed that phytohemagglutinin (PHA) rapidly enhances the uptake of potassium (K+) by human blood lymphocytes, and PHA produces a marked increase in the permeability of the lymphocyte membrane to K+, and the increase in active K+ influx in PHA-treated lymphocytes may represent a homeostatic response by the membrane K+ transport system to the increased in K+ efflux.
Exodus of 42K+ and 86Rb+ from rat thymic and human blood lymphocytes exposed to phytohemagglutinin
TLDR
86Rb+ efflux was increased by 27% in rat thymic lymphocytes and by 78% in human blood lymphocytes following PHA treatment, and the rate constant for efflux, Ke, was significantly increased by PHA.
Regulation of sodium and potassium transport in phytohemagglutinin-stimulated human blood lymphocytes.
TLDR
These studies indicate that the adaptive acceleration of Na and K transport in mitogen-treated lymphocytes is mediated by a small increase in cell Na, which is capable of increasing as the cell Na rises above normal.
Lymphocyte response to phytohemagglutinin: Intracellular volume and intracellular [K+]
TLDR
The decrease in lymphocyte water volume induced by PHA was also observed for concanavalin A which stimulates lymphocyte proliferation, but not for wheat germ lectin, an agglutinating agent which is not mitogenic, suggesting volume regulation may be closely associated with the mitogenicity of these compounds.
Early Biochemical Events in T-Lymphocyte Activation by Mitogens
TLDR
Central to the study of T- lymphocyte activation by mitogens has been the quest to attach causal significance to early changes and to establish a mitogen signal sequence leading to cellular replication.
Effect of ouabain on macromolecular synthesis during the cell cycle in mitogen-stimulated human lymphocytes.
TLDR
The results suggest that ouabain interferes with mitogen-triggered membrane-associated events, other than K+ transport, controlling mitosis at distinct phases of the cell cycle.
THE ROLE OF CATION FLUX IN TRIGGERING AND MAINTAINING THE STIMULATED STATE IN LYMPHOCYTES
Publisher Summary This chapter discusses the role of cation flux, in particular Ca ++ , K + , and Na + in lymphocyte transformation. Studies of the action of chelators and ionophores and of Ca ++
The effect of method on the measurement of K+ concentration in PHA-treated human blood lymphocytes.
TLDR
It is emphasized that disaggregated, lectin-treated lymphocytes suffer severe membrane damage and their contents cannot be expressed on the basis of cell number measured prior to washing with dispersion.
Decreased membrane potassium permeability and transport in human chronic leukemic and tonsillar lymphocytes
TLDR
The permeability and active transport of K+ by lymphocytes in chronic lymphocytic leukemia (CLL) was studied to determine if their membrane K+ transport was similar to resting or lectin‐stimulated normal blood lymphocytes.
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TLDR
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Exodus of 42K+ and 86Rb+ from rat thymic and human blood lymphocytes exposed to phytohemagglutinin
TLDR
86Rb+ efflux was increased by 27% in rat thymic lymphocytes and by 78% in human blood lymphocytes following PHA treatment, and the rate constant for efflux, Ke, was significantly increased by PHA.
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