Human lymphocyte potassium content during the initiation of phytohemagglutinin‐induced mitogenesis

  title={Human lymphocyte potassium content during the initiation of phytohemagglutinin‐induced mitogenesis},
  author={George B. Segel and Marshall A. Lichtman and Marsha M. Hollander and Bruce R. Gordon and Martin R. Klemperer},
  journal={Journal of Cellular Physiology},
The K+ content of human lymphocytes has been examined during the initial 24 hours after exposure of cells to phytohemagglutinin (PHA). We have reconfirmed that lymphocyte K+ exchanges rapidly for extracellular counterions during preparative washing if cells are exposed to PHA. By using a technique to measure cation content which does not require removal of cells from their culture medium, we have shown that K+ does not change for 24 hours following PHA treatment. Previous reports have… 
Publisher Summary This chapter focuses on transmembrane K+ turnover by phytohemagglutinin (PHA)-stimulated human lymphocytes. The alteration of the plasma membrane may be critical in the occurrence
Potasssium transport in human blood lymphocytes treated with phytohemagglutinin.
It is confirmed that phytohemagglutinin (PHA) rapidly enhances the uptake of potassium (K+) by human blood lymphocytes, and PHA produces a marked increase in the permeability of the lymphocyte membrane to K+, and the increase in active K+ influx in PHA-treated lymphocytes may represent a homeostatic response by the membrane K+ transport system to the increased in K+ efflux.
Exodus of 42K+ and 86Rb+ from rat thymic and human blood lymphocytes exposed to phytohemagglutinin
86Rb+ efflux was increased by 27% in rat thymic lymphocytes and by 78% in human blood lymphocytes following PHA treatment, and the rate constant for efflux, Ke, was significantly increased by PHA.
Regulation of sodium and potassium transport in phytohemagglutinin-stimulated human blood lymphocytes.
These studies indicate that the adaptive acceleration of Na and K transport in mitogen-treated lymphocytes is mediated by a small increase in cell Na, which is capable of increasing as the cell Na rises above normal.
Lymphocyte response to phytohemagglutinin: Intracellular volume and intracellular [K+]
The decrease in lymphocyte water volume induced by PHA was also observed for concanavalin A which stimulates lymphocyte proliferation, but not for wheat germ lectin, an agglutinating agent which is not mitogenic, suggesting volume regulation may be closely associated with the mitogenicity of these compounds.
Early Biochemical Events in T-Lymphocyte Activation by Mitogens
Central to the study of T- lymphocyte activation by mitogens has been the quest to attach causal significance to early changes and to establish a mitogen signal sequence leading to cellular replication.
Effect of ouabain on macromolecular synthesis during the cell cycle in mitogen-stimulated human lymphocytes.
The results suggest that ouabain interferes with mitogen-triggered membrane-associated events, other than K+ transport, controlling mitosis at distinct phases of the cell cycle.
Publisher Summary This chapter discusses the role of cation flux, in particular Ca ++ , K + , and Na + in lymphocyte transformation. Studies of the action of chelators and ionophores and of Ca ++
The effect of method on the measurement of K+ concentration in PHA-treated human blood lymphocytes.
It is emphasized that disaggregated, lectin-treated lymphocytes suffer severe membrane damage and their contents cannot be expressed on the basis of cell number measured prior to washing with dispersion.
Decreased membrane potassium permeability and transport in human chronic leukemic and tonsillar lymphocytes
The permeability and active transport of K+ by lymphocytes in chronic lymphocytic leukemia (CLL) was studied to determine if their membrane K+ transport was similar to resting or lectin‐stimulated normal blood lymphocytes.


A rapid phytohemagglutinin induced alteration in lymphocyte potassium permeability
Findings indicate that phytohemagglutinin produces a rapid alteration in lymphocyte plasma membranes so as to allow abnormal K+ exchange, which is of importance because investigators who measure intracellular solutes in phy tohemag GLUTinin‐treated lymphocytes must consider the possibility of loss during preparative washes.
Inhibition by Ouabain of Human Lymphocyte Transformation induced by Phytohaemagglutinin in vitro
In vitro ouabain, at relatively low concentrations, inhibits the blastogenic action of PHA as well as the incorporation of tritiated nucleosides into nucleic acid; excess potassium in the culture medium diminishes or prevents this effect of ouABain.
Exodus of 42K+ and 86Rb+ from rat thymic and human blood lymphocytes exposed to phytohemagglutinin
86Rb+ efflux was increased by 27% in rat thymic lymphocytes and by 78% in human blood lymphocytes following PHA treatment, and the rate constant for efflux, Ke, was significantly increased by PHA.
The early effects of ouabain on potassium metabolism and rate of proliferation of mouse lymphoblasts
Murine lymphoblasts grown in suspension culture in the presence of ouabain showed a dose dependent and sequential decrease in 86Rb+ (K+ analogue) influx, cellular potassium content, and growth rate, and inhibition was rapidly and completely reversible at concentrations that were not cytotoxic.
The effects of ouabain on the cell mitotic cycle of mouse lymphoblasts
The inhibition of cell proliferation by ouabain has been analyzed with respect to the cell cycle. Three lines of evidence indicate that growth rate is modified by altering to different degrees the
The monovalent cation content and adenosine triphosphatase activity of human normal and leukemic granulocytes and lymphocytes: relationship to cell volume and morphologic age.
The data are consistent with the hypothesis that increased sodium-potassium ATPase activity is a biochemical feature of leukocytes with the potential to divide and differentiate and are likely that discrepant values for leukocyte cation content and absence of monovalent cation activated ATPase previously reported are related to technical factors.
Cation Metabolism in Relation to Cell Size in Synchronously Grown Tissue Culture Cell
The pattern of electrolyte regulation during cell division does not follow a simple function such as cell number, cell surface, or cell volume, but must be related to specific internal events in the cell.
Early stimulation of potassium uptake in lymphocytes treated with PHA.
The results thus suggest that the categorization of lymphoid cells into the “short-lived” and the "long-lived" categories is a gross oversimplification, and are consistent with the interpretation that there are a multitude of subclasses of lymphoids cells, having discretely different life expectancies in the range from a few days or less to well over 1 month.
Intracellular Potassium and Macromolecular Synthesis in Mammalian Cells
The surface properties of cells may affect their ability to synthesize large molecules and the question whether the intracellular potassium concentration affects the synthesis of proteins and nucleic acids is raised.
The glomerular permeability determined by dextran clearance using Sephadex gel filtration.
  • C. Mogensen
  • Chemistry, Medicine
    Scandinavian journal of clinical and laboratory investigation
  • 1968
A method is described for measuring glomerular permeability by dextran clearance using Sephadex gel filtration technique. Clearance is shown to be a function of molecular size, decreasing with