Human lung project: evaluating variance of gene expression in the human lung.

@article{Gruber2006HumanLP,
  title={Human lung project: evaluating variance of gene expression in the human lung.},
  author={Michael P Gruber and Christopher D. Coldren and Malcolm D Woolum and Gregory P. Cosgrove and Chan Zeng and Anna E Baron and Mark D. Moore and Carlyne D Cool and George Scott Worthen and Kevin K. Brown and Mark W. Geraci},
  journal={American journal of respiratory cell and molecular biology},
  year={2006},
  volume={35 1},
  pages={65-71}
}
Nondiseased tissue is an important reference for microarray studies of pulmonary disease. We obtained 23 single lungs from multiorgan donors at time of procurement. Donors varied in age, sex, smoking history, and ethnicity. Lungs were dissected into upper and lower lobe peripheral sections for RNA extraction. Microarray analysis was performed using Affymetrix Hu-133 Plus 2.0 arrays. We observed that the relative variability of gene expression increased rapidly from technical (lowest), to… CONTINUE READING

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