Human lung microvessel endothelial cells: isolation, culture, and characterization.

@article{Hewett1993HumanLM,
  title={Human lung microvessel endothelial cells: isolation, culture, and characterization.},
  author={Peter W. Hewett and John C. Murray},
  journal={Microvascular research},
  year={1993},
  volume={46 1},
  pages={89-102}
}
The pulmonary vasculature is of great physiological/pathological significance. We have isolated and cultured microvessel endothelial cells (HuLEC) from lung tissue obtained from lung transplant recipients by modification of published methods. Pure cultures of HuLEC were isolated by mechanical disaggregation of the tissue prior to sequential dispase and trypsin digestion to obtain microvessel fragments. Magnetic beads (Dynabeads) coated with Ulex europaeus agglutinin-1 were then used to enhance… CONTINUE READING