Human lung membrane-bound neutral metallo-endopeptidase-catalyzed hydrolysis of bradykinin.

@article{Zolfaghari1989HumanLM,
  title={Human lung membrane-bound neutral metallo-endopeptidase-catalyzed hydrolysis of bradykinin.},
  author={R Zolfaghari and Darilyn Little and Chelsea R Baker and P C Canizaro and F J Bĕhal},
  journal={Enzyme},
  year={1989},
  volume={42 3},
  pages={160-73}
}
Human lung membrane-bound neutral metallo-endopeptidase (NME; EC 3.4.24.11) has been purified; this enzyme occurred in two forms, NME-I and NME-II. The total NME activity was purified 2,143-fold with the final specific activities for NME-I and NME-II being 750 and 1,124, respectively. The two NME forms were resolved in the final purification step involving ion exchange; in all earlier steps including gel filtration and affinity chromatography (phenyl sepharose) both forms behaved similarly and… CONTINUE READING