Human limbal mesenchymal cells support the growth of human corneal epithelial stem/progenitor cells.

@article{Nakatsu2014HumanLM,
  title={Human limbal mesenchymal cells support the growth of human corneal epithelial stem/progenitor cells.},
  author={Martin N. Nakatsu and Sheyla Gonz{\'a}lez and Hua Ping Mei and Sophie Xiaohui Deng Deng},
  journal={Investigative ophthalmology & visual science},
  year={2014},
  volume={55 10},
  pages={
          6953-9
        }
}
PURPOSE We tested the viability of human limbal mesenchymal cells (LMCs) to support the expansion of human corneal epithelial stem/progenitor cells (LSCs). METHODS Human LMCs were isolated from sclerocorneal tissue using collagenase A. Primary limbal epithelial cells (LECs) in the form of single cell suspension or cell clusters were cocultured on a monolayer of either 3T3 cells (control) or LMCs (SC-LMC culture). The LEC clusters also were grown directly on LMCs (CC-LMC culture) and in an… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
SHOWING 1-10 OF 18 CITATIONS

Similar Papers

Loading similar papers…