Human keratocyte migration into collagen gels declines with in vitro ageing
@article{Sandeman2000HumanKM, title={Human keratocyte migration into collagen gels declines with in vitro ageing}, author={Susan Sandeman and Marcus C. Allen and Christopher Liu and Richard G A Faragher and Andrew W. Lloyd}, journal={Mechanisms of Ageing and Development}, year={2000}, volume={119}, pages={149-157} }
22 Citations
The Transfer of Ocular Cells Using Collagen
- BiologyCell transplantation
- 2004
The results show that the cell seeding number must be critically balanced with the concentration of gel for it to be used as transplant material.
Insulin growth factor promotes human corneal fibroblast network formation in vitro.
- Biology, MedicineInvestigative ophthalmology & visual science
- 2011
In conclusion, IGF signaling and the MEK-1/2 pathway are involved in the cell network formation of corneal fibroblast cells from aged donors.
Online monitoring of the mechanical behavior of collagen hydrogels: influence of corneal fibroblasts on elastic modulus.
- Biology, EngineeringTissue engineering. Part C, Methods
- 2010
The ability to examine the mechanical properties of cell-seeded constructs in response to the culture environments--in particular, the response tothe addition of drugs or chemical reagents--which will provide a useful tool in studying the mechano-feedback loop between cells and their surrounding matrix is demonstrated.
Up-regulation of semaphorin 3A in human corneal fibroblasts by epidermal growth factor released from cocultured human corneal epithelial cells.
- Biology, MedicineBiochemical and biophysical research communications
- 2008
Cell Senescence Abrogates the Therapeutic Potential of Human Mesenchymal Stem Cells in the Lethal Endotoxemia Model
- Biology, MedicineStem cells
- 2014
It is shown that senescence induces extensive phenotypic changes in hMSCs and abrogates their protective activity in a murine model of LPS‐induced lethal endotoxemia and provides candidate gene signatures which may be useful to evaluate the therapeutic potential of h MSCs used in future clinical studies.
Multiple signaling pathways mediate compaction of collagen matrices by EGF-stimulated fibroblasts.
- BiologyExperimental cell research
- 2006
Aberrant heterodimerization of keratin 16 with keratin 6A in HaCaT keratinocytes results in diminished cellular migration
- BiologyMechanisms of Ageing and Development
- 2010
Cyclin D1 Overexpression Permits the Reproducible Detection of Senescent Human Vascular Smooth Muscle Cells
- BiologyAnnals of the New York Academy of Sciences
- 2007
Quantitative cytochemical analysis of senescent and growing vascular smooth muscle cells indicates that cyclin D1 reactivity is a considerably better marker of replicative senescence than senescENCE‐associated β‐galactosidase activity.
Electric signals regulated immunomodulation and wound healing
- Biology
- 2012
It is proposed that the EF-induced re-distribution of the receptors on the cell surface results in a shift of membrane receptors between the cathode-facing and the anodes-facing membrane of the cell, which would help to overcome the threshold of signal transduction at the higher density receptor side.
Cellular senescence, ageing and disease
- Biology, MedicineAGE
- 2008
Recent findings which suggest that cellular senescence does contribute to ageing and the development/progression of disease are discussed.
References
SHOWING 1-10 OF 24 REFERENCES
Fibroblast migration and proliferation during in vitro wound healing
- Biology, MedicineResearch in experimental medicine. Zeitschrift fur die gesamte experimentelle Medizin einschliesslich experimenteller Chirurgie
- 1993
During the formation of granulation tissue in a dermal wound, plate-lets, monocytes and other cellular blood constituents release various peptide growth factors to stimulate fibroblasts to migrate…
Epithelial injury induces keratocyte apoptosis: hypothesized role for the interleukin-1 system in the modulation of corneal tissue organization and wound healing.
- Medicine, BiologyExperimental eye research
- 1996
The epithelial/endothelial-stromal IL-1 system may mediate corneal tissue organization and responses to mechanical- and pathogen-induced injury through induction of keratocyte apoptosis and it is hypothesize that derangement's in this system may have a role in the pathogenesis of ker atoconus and other diseases of the cornea.
Keratocyte migration and peptide growth factors: the effect of PDGF, bFGF, EGF, IGF-I, aFGF and TGF-beta on human keratocyte migration in a collagen gel.
- Medicine, BiologyCurrent eye research
- 1997
PDGF, EGF and bFGF increase keratocyte migration, independent of proliferation in a collagen gel invasion assay and might promote corneal wound healing, not only by increasing cell proliferation, but also through increased motility.
Interaction of fibronectin with collagen: age-specific defect in the biological activity of human fibroblast fibronectin.
- Biology, MedicineProceedings of the National Academy of Sciences of the United States of America
- 1983
Comparisons of molecular weights by NaDodSO4/polyacrylamide gel electrophoresis reveals that fibronectin from late-passaging cells is larger than that from early-passage cells, which supports the hypothesis that late-Passage cells produce a structurally and functionally distinct fibronECTin.
A Standard Strain of Human Ocular Keratocytes
- Medicine, BiologyOphthalmic Research
- 1998
A strain of human embryonic keratocytes, designated EK1.BR, has been reported as a research tool for the ophthalmic community and it is hoped that these cells will prove useful in the in vitro study of stromal wound healing and the characterisation of keratocyte gene expression.
Increased organization of cytoskeleton accompanying the aging of human fibroblasts in vitro.
- BiologyExperimental cell research
- 1984
Control of cell proliferation in the human embryonic cornea: an autoradiographic analysis of the effect of growth factors on DNA synthesis in endothelial and stromal cells in organ culture and after explantation in vitro.
- Biology, MedicineJournal of cell science
- 1986
Results suggest that the proliferation of corneal endothelial cells and stromal cells is dependent on EGF-like factors as well as on some insulin-like substance during embryogenesis.
Similar, but not identical, modulation of expression of extracellular matrix components during in vitro and in vivo aging of human skin fibroblasts
- Medicine, BiologyJournal of cellular physiology
- 1992
It is suggested that the decreased synthesis of procollagen and proteoglycans in in vivo aged fibroblasts represents changes that are responsible for intrinsic degenerative changes that occur in human skin during aging.
Aging and the cornea
- MedicineThe British journal of ophthalmology
- 1997
It is helpful to consider corneal aging alterations within the broader framework of the aging process, as it is difficult to distinguish age specific deterioration from degenerations modified by environmental and genetic factors.
β-Galactosidase histochemistry and telomere loss in senescent retinal pigment epithelial cells
- Biology
- 1999
Senescence-related beta-galactosidase activity and mean TRF length may be valuable in determining senescence of the retinal pigment epithelium in vivo, where senescent RPE cells could be involved in the development of age-related maculopathy and age- related macular degeneration.