Human keratocyte migration into collagen gels declines with in vitro ageing

@article{Sandeman2000HumanKM,
  title={Human keratocyte migration into collagen gels declines with in vitro ageing},
  author={Susan Sandeman and Marcus C. Allen and Christopher Liu and R G A Faragher and Andrew W. Lloyd},
  journal={Mechanisms of Ageing and Development},
  year={2000},
  volume={119},
  pages={149-157}
}
Although senescence in various cell types has been shown to have detrimental effects on wound repair, the effect of this phenomenon on corneal function with increasing age has yet to be elucidated. This study investigated the effect of in vitro ageing on keratocyte migration into a collagen gel matrix. The keratocyte cell strain EK1. BR was cultured to late passage and a comparison of early passage migration with that of late passage migration was carried out. Early or late passage keratocytes… 
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    References

    SHOWING 1-10 OF 24 REFERENCES
    Fibroblast migration and proliferation during in vitro wound healing
    • T. Schreier, E. Degen, W. Baschong
    • Biology, Medicine
      Research in experimental medicine. Zeitschrift fur die gesamte experimentelle Medizin einschliesslich experimenteller Chirurgie
    • 1993
    During the formation of granulation tissue in a dermal wound, plate-lets, monocytes and other cellular blood constituents release various peptide growth factors to stimulate fibroblasts to migrate…
    Epithelial injury induces keratocyte apoptosis: hypothesized role for the interleukin-1 system in the modulation of corneal tissue organization and wound healing.
    TLDR
    The epithelial/endothelial-stromal IL-1 system may mediate corneal tissue organization and responses to mechanical- and pathogen-induced injury through induction of keratocyte apoptosis and it is hypothesize that derangement's in this system may have a role in the pathogenesis of ker atoconus and other diseases of the cornea.
    Keratocyte migration and peptide growth factors: the effect of PDGF, bFGF, EGF, IGF-I, aFGF and TGF-beta on human keratocyte migration in a collagen gel.
    TLDR
    PDGF, EGF and bFGF increase keratocyte migration, independent of proliferation in a collagen gel invasion assay and might promote corneal wound healing, not only by increasing cell proliferation, but also through increased motility.
    Interaction of fibronectin with collagen: age-specific defect in the biological activity of human fibroblast fibronectin.
    TLDR
    Comparisons of molecular weights by NaDodSO4/polyacrylamide gel electrophoresis reveals that fibronectin from late-passaging cells is larger than that from early-passage cells, which supports the hypothesis that late-Passage cells produce a structurally and functionally distinct fibronECTin.
    A Standard Strain of Human Ocular Keratocytes
    TLDR
    A strain of human embryonic keratocytes, designated EK1.BR, has been reported as a research tool for the ophthalmic community and it is hoped that these cells will prove useful in the in vitro study of stromal wound healing and the characterisation of keratocyte gene expression.
    Increased organization of cytoskeleton accompanying the aging of human fibroblasts in vitro.
    TLDR
    Results suggest that during in vitro senescence, the increase in cell size is correlated with increased organization of the cytoskeleton, and the presence of a rigid cytoskeletal structure may contribute in part to the inability of the senescent cell to replicate.
    Control of cell proliferation in the human embryonic cornea: an autoradiographic analysis of the effect of growth factors on DNA synthesis in endothelial and stromal cells in organ culture and after explantation in vitro.
    • L. Hyldahl
    • Biology, Medicine
      Journal of cell science
    • 1986
    TLDR
    Results suggest that the proliferation of corneal endothelial cells and stromal cells is dependent on EGF-like factors as well as on some insulin-like substance during embryogenesis.
    Similar, but not identical, modulation of expression of extracellular matrix components during in vitro and in vivo aging of human skin fibroblasts
    TLDR
    It is suggested that the decreased synthesis of procollagen and proteoglycans in in vivo aged fibroblasts represents changes that are responsible for intrinsic degenerative changes that occur in human skin during aging.
    Aging and the cornea
    TLDR
    It is helpful to consider corneal aging alterations within the broader framework of the aging process, as it is difficult to distinguish age specific deterioration from degenerations modified by environmental and genetic factors.
    β-Galactosidase histochemistry and telomere loss in senescent retinal pigment epithelial cells
    TLDR
    Senescence-related beta-galactosidase activity and mean TRF length may be valuable in determining senescence of the retinal pigment epithelium in vivo, where senescent RPE cells could be involved in the development of age-related maculopathy and age- related macular degeneration.
    ...
    1
    2
    3
    ...