Human intraspecific somatic cell hybrids: a genetic and karyotypic analysis of crosses between lymphocytes and D98/AH-2.

@article{Bengtsson1975HumanIS,
  title={Human intraspecific somatic cell hybrids: a genetic and karyotypic analysis of crosses between lymphocytes and D98/AH-2.},
  author={Bengt Olle Bengtsson and Markus Nabholz and Roger Kennett and Walter F. Bodmer and Sue Povey and Dallas Swallow},
  journal={Somatic cell genetics},
  year={1975},
  volume={1 1},
  pages={41-64}
}
A number of human intraspecific hybrids were produced by fusing the 8-azaguanine-resistant cell line D98/AH-2 with PHA-stimulated lymphocytes from a normal human male, followed by selection in HAT medium. The parent cells differed in zymogram patterns for 4 enzyme systems. Hypoxanthine-guanine phophoribosyltransferase was missing in D98/AH-2 and was determined in the hybrids by the normal gene derived from the lymphocyte donor's X chromosome. The HL-A antigens of the lymphocyte donor as well as… CONTINUE READING