Human intestinal alkaline phosphatase-binding IgG in patients with severe bacterial infections.

@article{Mder1994HumanIA,
  title={Human intestinal alkaline phosphatase-binding IgG in patients with severe bacterial infections.},
  author={Michael M{\"a}der and N Kolbus and D Meihorst and Andreas K{\"o}hn and Wolfgang Beuche and Klaus Felgenhauer},
  journal={Clinical and experimental immunology},
  year={1994},
  volume={95 1},
  pages={98-102}
}
Patterns of alkaline phosphatase (AP)-binding proteins were observed in the alkaline pH range of 6.5-9.5 upon isoelectric focusing and blotting of serum from patients with inflammatory diseases. After isolation using affinity chromatography on protein A or immunoaffinity chromatography on AP coupled to cyanogen bromide (CNBr)-activated Sepharose, the AP-binding protein was identified as IgG on Western blots and in ELISA using human IgG-specific antibodies. It was shown that this IgG binds to AP… CONTINUE READING