Human intervertebral disc aggrecan inhibits nerve growth in vitro.

  title={Human intervertebral disc aggrecan inhibits nerve growth in vitro.},
  author={William Johnson and Bruce Caterson and Stephen M. Eisenstein and Dianna L Hynds and Diane M. Snow and Sally Roberts},
  journal={Arthritis and rheumatism},
  volume={46 10},
OBJECTIVE To assess the effects of human intervertebral disc aggrecan on nerve growth and guidance, using in vitro techniques. METHODS Aggrecan extracted from human lumbar intervertebral discs was incorporated into tissue culture substrata for the culture of the human neuronal cell line, SH-SY5Y, or explants of chick dorsal root ganglia. The effects on nerve growth of different concentrations of aggrecan extracted from the anulus fibrosus and nucleus pulposus, and of these aggrecan… CONTINUE READING