Human insulin-degrading enzyme working mechanism.

  title={Human insulin-degrading enzyme working mechanism.},
  author={Orazio Amata and T. Marino and N. Russo and M. Toscano},
  journal={Journal of the American Chemical Society},
  volume={131 41},
  • Orazio Amata, T. Marino, +1 author M. Toscano
  • Published 2009
  • Chemistry, Medicine
  • Journal of the American Chemical Society
  • The possible mechanism by which the insulin-degrading enzyme (IDE) zinc-binding protease carries out its catalytic function toward two peptides of different length, simulating a portion of B chain of insulin, was investigated on an enzymatic model consisting of 130 /159 atoms, using the density functional theory method and the hybrid exchange-correlation functional B3LYP in gas phase and in the protein environment. Based on the geometry and relative stabilities of minima and transition states… CONTINUE READING
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