Human immunodeficiency virus type 1 enters brain microvascular endothelia by macropinocytosis dependent on lipid rafts and the mitogen-activated protein kinase signaling pathway.

@article{Liu2002HumanIV,
  title={Human immunodeficiency virus type 1 enters brain microvascular endothelia by macropinocytosis dependent on lipid rafts and the mitogen-activated protein kinase signaling pathway.},
  author={Nancy Liu and Albert Lossinsky and Waldemar Popik and Xia Li and Chandrasekhar Gujuluva and Benjamin M Kriederman and Jaclyn Landau Roberts and Tatania Pushkarsky and Michael I. Bukrinsky and Marlys Witte and Martin E. Weinand and Milan Fiala},
  journal={Journal of virology},
  year={2002},
  volume={76 13},
  pages={6689-700}
}
Brain microvascular endothelial cells (BMVECs) present an incomplete barrier to human immunodeficiency virus type 1 (HIV-1) neuroinvasion. In order to clarify the mechanisms of HIV-1 invasion, we have examined HIV-1 uptake and transcellular penetration in an in vitro BMVEC model. No evidence of productive infection was observed by luciferase, PCR, and reverse transcriptase assays. Approximately 1% of viral RNA and 1% of infectious virus penetrated the BMVEC barrier without disruption of tight… CONTINUE READING