Human hematopoietic stem / progenitor cells modified by zinc-finger nucleases targeted to CCR 5 control HIV-1 in vivo

@inproceedings{Holt2010HumanHS,
  title={Human hematopoietic stem / progenitor cells modified by zinc-finger nucleases targeted to CCR 5 control HIV-1 in vivo},
  author={Nathalia G Holt and Jianbin Wang and Kenneth Eunhan Kim and Geoffrey Friedman and Xingchao Wang and Vanessa Taupin and Gay M. Crooks and Donald B Kohn and Philip D. Gregory and Michael C. Holmes and Paula M. Cannon},
  year={2010}
}
CCR5 is the major HIV-1 co-receptor, and individuals homozygous for a 32-bp deletion in CCR5 are resistant to infection by CCR5-tropic HIV-1. Using engineered zinc-finger nucleases (ZFNs), we disrupted CCR5 in human CD34+ hematopoietic stem/ progenitor cells (HSPCs) at a mean frequency of 17% of the total alleles in a population. This procedure produces both monoand bi-allelically disrupted cells. ZFN-treated HSPCs retained the ability to engraft NOD/SCID/IL2rγnull mice and gave rise to… CONTINUE READING
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IFN - alpha - induced upregulation of CCR 5 leads to expanded HIV tropism in vivo

  • S. K. Choudhary
  • PLoS Pathog .
  • 2010

- derived CD 34 + hematopoietic stem and progenitor cells by a hyperactive Sleeping Beauty transposon system

  • S. Basu, H. e. Broxmeyer
  • 2009

Gene therapy for immunodeficiency due to adenosine deaminase deficiency

  • A. Aiuti
  • N . Engl . J . Med .
  • 2009

Hematopoietic stem cell gene therapy with a lentiviral vector in X - linked adrenoleukodystrophy

  • N. Cartier
  • Science
  • 2009

Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates

  • X. Xue
  • Nat . Genet .
  • 2009

Novel targets for antiretroviral therapy : clinical progress to date

  • G. Hutter
  • Drugs
  • 2009

Phase 2 gene therapy trial of an anti - HIV ribozyme in autologous CD 34 + cells

  • R. t. Mitsuyasu
  • Nat . Med .
  • 2009

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