Human granulosa cells synthesize low molecular weight insulin-like growth factor-binding protein.

Abstract

Human follicular fluid contains insulin-like growth factor I (IGF-I) and its low molecular weight binding protein (IGF-BP). We studied the synthesis of IGF-BP by the granulosa cells obtained after ovarian hyperstimulation for in vitro fertilization. The granulosa cells were cultured for 72 hours in Ham's F-10 medium supplemented with 10% fetal calf serum (FCS). Samples of the culture medium were collected every 24 hours. The IGF-BP concentration in culture medium increased from 1.2 to 2.1 micrograms/l at 48 h and to 3.3 micrograms/l at 72 h. De novo synthesis of IGF-BP was shown by incorporation of labeled methionine into immunoreactive IGF-BP, as detected by SDS polyacrylamide electrophoresis (PAGE) and fluorography. Our results demonstrate synthesis of IGF-BP in the human ovary.

Cite this paper

@article{Suikkari1989HumanGC, title={Human granulosa cells synthesize low molecular weight insulin-like growth factor-binding protein.}, author={A M Suikkari and Jari Juhani Jalkanen and Riitta A Koistinen and Ralf B{\"{u}tzow and Olli V-P. Ritvos and Tapio Ranta and Markku Sepp{\"a}l{\"a}}, journal={Endocrinology}, year={1989}, volume={124 2}, pages={1088-90} }