Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts.

@article{Schnieke1997HumanFI,
  title={Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts.},
  author={Angelika Schnieke and Alexander J. Kind and William A. Ritchie and Katherine Mycock and Ann R. Scott and Marjorie Ritchie and Ian Wilmut and Alan Colman and Keith Henry Stockman Campbell},
  journal={Science},
  year={1997},
  volume={278 5346},
  pages={
          2130-3
        }
}
Ovine primary fetal fibroblasts were cotransfected with a neomycin resistance marker gene (neo) and a human coagulation factor IX genomic construct designed for expression of the encoded protein in sheep milk. Two cloned transfectants and a population of neomycin (G418)-resistant cells were used as donors for nuclear transfer to enucleated oocytes. Six transgenic lambs were liveborn: Three produced from cloned cells contained factor IX and neo transgenes, whereas three produced from the… 
Production of Transgenic Goats, Carrying Human Coagulation Factor IX cDNA, by Nuclear Transfer of Transfected Fetal Fibroblasts
TLDR
This study has provided the groundwork for a prosperous and promising approach for large-scale production and therapeutic application of hFIX expressed in transgenic goats.
Production of transgenic goats expressing human coagulation factor IX in the mammary glands after nuclear transfer using transfected fetal fibroblast cells
TLDR
This study has provided the groundwork for a prosperous and promising approach for large-scale production and therapeutic application of hFIX expressed in transgenic goats.
Production of gene-targeted sheep by nuclear transfer from cultured somatic cells
TLDR
Efficient and reproducible gene targeting in fetal fibroblasts to place a therapeutic transgene at the ovine α1(I) procollagen (COL1A1) locus is described and the production of live sheep by nuclear transfer is described.
Cloned pigs generated from cultured skin fibroblasts derived from a H‐transferase transgenic boar
TLDR
Cloned pigs were produced from cultured skin fibroblasts derived from a H‐transferase transgenic boar and PCR and FACS analysis determined that the cloned offspring contained and expressed the H‐ transferase transgene.
Nuclear transfer of goat somatic cells transgenic for human lactoferrin gene
TLDR
An efficient gene transfer and nuclear transfer approach is established in goat somatic cells to produce mammary gland bioreactors and some of reconstructed embryos could develop into blastocyst in vitro.
Production of transgenic calves by somatic cell nuclear transfer
TLDR
The results suggest that transgenic calves could be efficiently produced by SCNT using transgenic cells as nuclei donor and all cloned animals could be ensured to be transgenic by efficiently pre-screening transgenic Cells and SCNT embryos using the constructed double marker selective vector.
Production of cloned goats by nuclear transfer of cumulus cells and long‐term cultured fetal fibroblast cells into abattoir‐derived oocytes
TLDR
Cloned goats were produced by NT from cumulus cells and long‐term cultured fetal fibroblast cells to abattoir‐derived oocytes and microsatellite analysis of 10 markers confirmed that all cloned offspring were derived from corresponding donor cells.
Transgene expression of enhanced green fluorescent protein in cloned rabbits generated from in vitro-transfected adult fibroblasts
TLDR
It is demonstrated that transgenic rabbits can be generated through nuclear transfer and that all 4 cloned rabbits were genetically identical to the transgenic donor cells, and that they all carried the EGFP gene.
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References

SHOWING 1-10 OF 28 REFERENCES
Characterization of recombinant human factor IX expressed in transgenic mice and in derived trans‐immortalized hepatic cell lines.
TLDR
By crossing transgenic mice synthesizing FIX with others prone to develop hepatoma, progeny which co‐express the transgenes in hepatocytes were obtained and Hepatoma‐derived cell lines were shown to have a differentiated phenotype and secrete active human FIX for many generations.
Transgenic cattle resulting from biopsied embryos: expression of c-ski in a transgenic calf.
TLDR
A system for early identification of transgenic embryos in which biopsies of embryos were assayed by polymerase chain reaction for presence of the transgene before embryo transfer resulted in production of 29 putative transgenic fetuses and one calf, which was subsequently shown to be definitively transgenic.
Sheep cloned by nuclear transfer from a cultured cell line
TLDR
This is the first report, to the authors' knowledge, of live mammalian offspring following nuclear transfer from an established cell line, and will provide the same powerful opportunities for analysis and modification of gene function in livestock species that are available in the mouse through the use of embryonic stem cells.
Production of transgenic rabbits, sheep and pigs by microinjection
TLDR
Integration of the fusion gene in all three species and expression of the gene in transgenic rabbits and pigs are reported here.
Production of cloned lambs from an established embryonic cell line: a comparison between in vivo- and in vitro-matured cytoplasts.
TLDR
DNA microsatellite markers conclusively show that the three lambs are genetically identical and were derived from the embryonic cell line, and some cells from this blastocyst-derived embryonic cellline are totipotent by nuclear transfer and can produce viable offspring.
Transgenic Livestock as Bioreactors: Stable Expression of Human Alpha-1-Antitrypsin by a Flock of Sheep
TLDR
Although the inheritance of the same transgene in mice was reminiscent of the situation in sheep, stable expression was observed in only one of four lines studied, and the importance of these observations to the use of transgenic livestock as bioreactors for the production of human proteins is discussed.
Improved Wool Production in Transgenic Sheep Expressing Insulin-like Growth Factor 1
TLDR
To the authors' knowledge this is the first reported improvement in a production trait by genetic engineering of a farm animal without adverse effects on health or reproduction.
Development and validation of swine embryonic stem cells: a review.
  • M. Wheeler
  • Biology
    Reproduction, fertility, and development
  • 1994
TLDR
Porcine (Sus scrofa) embryonic stem (ES) cell lines from preimplantation blastocysts and their ability to develop into normal chimaeras are summarized, indicating a pluripotent cell.
Pluripotent bovine embryonic cell lines direct embryonic development following nuclear transfer.
TLDR
Blastocyst- and morula-derived embryonic cell line nuclei are pluripotent in that they can direct development through organogenesis, with subsequent pregnancy loss due, at least in part, to a deficiency in placentome development.
Fixing human factor IX (fIX): correction of a cryptic RNA splice enables the production of biologically active fIX in the mammary gland of transgenic mice.
  • F. Yull, G. Harold, A. Clark
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1995
TLDR
The purified protein is fully active showing that the mammary gland is capable of the efficient post-translational modification of this protein and that transgenic animals are a suitable means of its production.
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