Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts.

@article{Schnieke1997HumanFI,
  title={Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts.},
  author={Angelika Schnieke and Alexander J. Kind and William A. Ritchie and Katherine Mycock and Ann R. Scott and Marjorie Ritchie and Ian Wilmut and Alan Colman and Keith Henry Stockman Campbell},
  journal={Science},
  year={1997},
  volume={278 5346},
  pages={
          2130-3
        }
}
Ovine primary fetal fibroblasts were cotransfected with a neomycin resistance marker gene (neo) and a human coagulation factor IX genomic construct designed for expression of the encoded protein in sheep milk. Two cloned transfectants and a population of neomycin (G418)-resistant cells were used as donors for nuclear transfer to enucleated oocytes. Six transgenic lambs were liveborn: Three produced from cloned cells contained factor IX and neo transgenes, whereas three produced from the… 
Production of Transgenic Goats, Carrying Human Coagulation Factor IX cDNA, by Nuclear Transfer of Transfected Fetal Fibroblasts
TLDR
This study has provided the groundwork for a prosperous and promising approach for large-scale production and therapeutic application of hFIX expressed in transgenic goats.
Production of transgenic goats expressing human coagulation factor IX in the mammary glands after nuclear transfer using transfected fetal fibroblast cells
TLDR
This study has provided the groundwork for a prosperous and promising approach for large-scale production and therapeutic application of hFIX expressed in transgenic goats.
Production of gene-targeted sheep by nuclear transfer from cultured somatic cells
TLDR
Efficient and reproducible gene targeting in fetal fibroblasts to place a therapeutic transgene at the ovine α1(I) procollagen (COL1A1) locus is described and the production of live sheep by nuclear transfer is described.
Cloned pigs generated from cultured skin fibroblasts derived from a H‐transferase transgenic boar
TLDR
Cloned pigs were produced from cultured skin fibroblasts derived from a H‐transferase transgenic boar and PCR and FACS analysis determined that the cloned offspring contained and expressed the H‐ transferase transgene.
Nuclear transfer of goat somatic cells transgenic for human lactoferrin gene
TLDR
An efficient gene transfer and nuclear transfer approach is established in goat somatic cells to produce mammary gland bioreactors and some of reconstructed embryos could develop into blastocyst in vitro.
Production of transgenic calves by somatic cell nuclear transfer
TLDR
The results suggest that transgenic calves could be efficiently produced by SCNT using transgenic cells as nuclei donor and all cloned animals could be ensured to be transgenic by efficiently pre-screening transgenic Cells and SCNT embryos using the constructed double marker selective vector.
Production of cloned goats by nuclear transfer of cumulus cells and long‐term cultured fetal fibroblast cells into abattoir‐derived oocytes
TLDR
Cloned goats were produced by NT from cumulus cells and long‐term cultured fetal fibroblast cells to abattoir‐derived oocytes and microsatellite analysis of 10 markers confirmed that all cloned offspring were derived from corresponding donor cells.
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TLDR
By crossing transgenic mice synthesizing FIX with others prone to develop hepatoma, progeny which co‐express the transgenes in hepatocytes were obtained and Hepatoma‐derived cell lines were shown to have a differentiated phenotype and secrete active human FIX for many generations.
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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