Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells

@article{Ullrich1984HumanEG,
  title={Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells},
  author={Axel Ullrich and Lisa M. Coussens and Joel S. Hayflick and Thomas J. Dull and Alane M. Gray and A. W. Tam and J. Lee and Yosef Yarden and Towia A Libermann and Joseph Schlessinger and Julian Downward and Elaine L. V. Mayes and N. Whittle and Michael D. Waterfield and P. H. Seeburg},
  journal={Nature},
  year={1984},
  volume={309},
  pages={418-425}
}
The complete 1,210-amino acid sequence of the human epidermal growth factor (EGF) receptor precursor, deduced from cDNA clones derived from placental and A431 carcinoma cells, reveals close similarity between the entire predicted ν-erb-B mRNA oncogene product and the receptor transmembrane and cytoplasmic domains. A single transmembrane region of 23 amino acids separates the extracellular EGF binding and cytoplasmic domains. The receptor gene is amplified and apparently rearranged in A431 cells… 

A 1.8 kb alternative transcript from the human epidermal growth factor receptor gene encodes a truncated form of the receptor.

It is demonstrated that alternative processing of the human EGFR transcript produces a secreted product composed of only the extracellular ligand-binding domain.

Human epidermal growth factor receptor cDNA is homologous to a variety of RNAs overproduced in A431 carcinoma cells

The recently discovered similarity between the human epidermal growth factor (EGF) receptor and the avian erythroblastosis virus v-erb-B protein1 supports the hypothesis that viral oncogenes share a

Amplified gene for the epidermal growth factor receptor in a human glioblastoma cell line encodes an enzymatically inactive protein

The gene encoding the receptor for epidermal growth factor was amplified two- to fivefold in the human glioblastoma cell line SF268 and it is presumed that the amplified receptor gene carries a mutation(s) that affects several aspects of the receptor's function.

Amplification of the structurally and functionally altered epidermal growth factor receptor gene (c-erbB) in human brain tumors

By using Southern blot analysis, we found that in two cases of human glioblastoma multiforme, cells carried amplified c-erbB genes which bore short deletion mutations within the ligand-binding domain

Relationship between production of epidermal growth factor receptors, gene amplification, and chromosome 7 translocation in variant A431 cells

Gene amplification as the mechanism enhancing A431 cell EFG receptor protein and determining growth responses is supported, and correlation existed in variants selected for reduced EGF receptors and in revenants from those variants with increased E GF receptors.

Amplification of a novel v-erbB-related gene in a human mammary carcinoma.

A new member of the tyrosine kinase proto-oncogene family has been identified on the basis of its amplification in a human mammary carcinoma.

A truncated, secreted form of the epidermal growth factor receptor is encoded by an alternatively spliced transcript in normal rat tissue

Southern blot analysis of rat genomic DNA indicated that the 3'-terminal sequence of this transcript is derived from the EGF-R gene, and Northern (RNA blot) analysis established that the truncated receptor is encoded by a 2.7-kb mRNA which codes for a truncated form of the receptor.

Selective amplification of the cytoplasmic domain of the epidermal growth factor receptor gene in glioblastoma multiforme.

Primary brain tumors of glial origin often overexpress epidermal growth factor receptors (EGF-Rs) and this may be associated with amplification of the EGF-R gene, which is found in four glioblastoma multiforme tumors.

Structure and localization of genes encoding aberrant and normal epidermal growth factor receptor RNAs from A431 human carcinoma cells

In situ hybridization to chromosomes from normal cells and A431 cells show that both the EGF receptor gene and the unidentified DNA are localized to the p14-p12 region of chromosome 7.
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