Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells

@article{Ullrich1984HumanEG,
  title={Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells},
  author={Axel Ullrich and Lisa M. Coussens and Joel S. Hayflick and Thomas J. Dull and Alane M. Gray and A. W. Tam and J. Lee and Yosef Yarden and Towia A Libermann and Joseph Schlessinger and Julian Downward and Elaine L. V. Mayes and N. Whittle and Michael D. Waterfield and P. H. Seeburg},
  journal={Nature},
  year={1984},
  volume={309},
  pages={418-425}
}
The complete 1,210-amino acid sequence of the human epidermal growth factor (EGF) receptor precursor, deduced from cDNA clones derived from placental and A431 carcinoma cells, reveals close similarity between the entire predicted ν-erb-B mRNA oncogene product and the receptor transmembrane and cytoplasmic domains. A single transmembrane region of 23 amino acids separates the extracellular EGF binding and cytoplasmic domains. The receptor gene is amplified and apparently rearranged in A431 cells… 
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Relationship between production of epidermal growth factor receptors, gene amplification, and chromosome 7 translocation in variant A431 cells

Gene amplification as the mechanism enhancing A431 cell EFG receptor protein and determining growth responses is supported, and correlation existed in variants selected for reduced EGF receptors and in revenants from those variants with increased E GF receptors.

Amplification of a novel v-erbB-related gene in a human mammary carcinoma.

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A truncated, secreted form of the epidermal growth factor receptor is encoded by an alternatively spliced transcript in normal rat tissue

Southern blot analysis of rat genomic DNA indicated that the 3'-terminal sequence of this transcript is derived from the EGF-R gene, and Northern (RNA blot) analysis established that the truncated receptor is encoded by a 2.7-kb mRNA which codes for a truncated form of the receptor.

Selective amplification of the cytoplasmic domain of the epidermal growth factor receptor gene in glioblastoma multiforme.

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Isolation of an evolutionarily conserved epidermal growth factor receptor cDNA from human A431 carcinoma cells.

Structure and localization of genes encoding aberrant and normal epidermal growth factor receptor RNAs from A431 human carcinoma cells

In situ hybridization to chromosomes from normal cells and A431 cells show that both the EGF receptor gene and the unidentified DNA are localized to the p14-p12 region of chromosome 7.
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References

SHOWING 1-10 OF 54 REFERENCES

Close similarity of epidermal growth factor receptor and v-erb-B oncogene protein sequences

Each of six peptides derived from the human epidermal growth factor (EGF) receptor very closely matches a part of the deduced sequence of the v-erb-B transforming protein of avian erythroblastosis…

Amplification and enhanced expression of the epidermal growth factor receptor gene in A431 human carcinoma cells.

Results are consistent with the hypothesis that, in A431 cells, gene amplification, possibly associated with a translocation event, may result in the overproduction of EGF receptor protein or the appearance of the transformed phenotype (or both).

Production of an epidermal growth factor receptor-related protein.

Human epidermoid carcinoma A431 cells in culture produce a soluble 105-kilodalton protein which, by the criteria of epidermal growth factor (EGF) binding, recognition by monoclonal and polyclonal…

Nucleotide sequence of epidermal growth factor cDNA predicts a 128,000-molecular weight protein precursor

The data indicate that the discrepancy between EGF levels in male and female mouse submaxillary glands (MSGs) is due to different EGF mRNA levels in these tissues, and suggest that precursor EGF processing may differ from that described previously for other polypeptide hormones.

Genetic analysis of epidermal growth factor action: assignment of human epidermal growth factor receptor gene to chromosome 7.

The results suggest that the structural gene or a gene necessary for expression of the human EGF receptor is located on human chromosome 7.

Identification of epidermal growth factor receptors in a hyperproducing human epidermoid carcinoma cell line.

The loss of EGF binding activity by cells during down regulation closely paralleled the decrease in receptor subject to direct labeling and the decreases in the 175,000-dalton band labeled by surface-specific radioiodination.

The membrane glycoprotein encoded by the retroviral oncogene v-erb-B is structurally related to tyrosine-specific protein kinases.

It is reported here that the v-erb B glycoprotein shares strong structural homologies with the tyrosine-specific protein kinases encoded by certain retroviral oncogenes.

Monoclonal antibodies against receptor for epidermal growth factor induce early and delayed effects of epidermal growth factor.

Observations support the notion that the biological information of the EGF-receptor complex resides in the membrane receptor, and the antibodies offer a powerful tool to study the structure, processing, and mode of action of EGF receptors.

Biosynthesis of the epidermal growth factor receptor in A431 cells.

A monoclonal antibody R1 against the human epidermal growth factor receptor has been used to study biosynthesis in the carcinoma cell line A431 to establish a precursor role for the 95 000 mol.

The erbB gene of avian erythroblastosis virus is a member of the src gene family

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