Human eosinophil cytotoxicity-enhancing factor. Purification, physical characteristics, and partial amino acid sequence of an active polypeptide.

@article{Silberstein1989HumanEC,
  title={Human eosinophil cytotoxicity-enhancing factor. Purification, physical characteristics, and partial amino acid sequence of an active polypeptide.},
  author={David S. Silberstein and Mayssam H Ali and Shannon L Baker and John R. David},
  journal={Journal of immunology},
  year={1989},
  volume={143 3},
  pages={979-83}
}
Medium conditioned by PMA/LPS-stimulated U937 cells was processed for the purification of an eosinophil cytotoxicity-enhancing factor (ECEF) by the following sequence: 1) phenyl-Sepharose chromatography; 2) DEAE-cartridge chromatography; 3) preparative SDS-gel electrophoresis; and 4) reversed-phase HPLC. This resulted in the isolation of a 10 kDa… CONTINUE READING