Human embryonic stem cells: origin, properties and applications

  title={Human embryonic stem cells: origin, properties and applications},
  author={Henrik Semb},
  • H. Semb
  • Published 1 November 2005
  • Biology
Human embryonic stem cells originate from the human preimplantation embryo. The derivation of the first human embryonic stem cells was reported in 1998. Since then we have learnt a great deal about how to isolate and culture these cells. Additionally, their stem cell phenotype and differentiation competence have been determined. Although it is expected that many basic biological properties, such as self‐renewal and cell specification, are evolutionary conserved, at least from the mouse, we lack… 


The aim of this study was to test different components with a house-made medium developed for hESC in order to improve the current formulation and the most promising additives proved to be amino acids, thiamine and glutathione.

Establishment and characterization of human embryonic stem cell lines, Turkey perspectives

The characteristic of hESC lines, which were established in Istanbul Memorial Hospital between 2003 and 2005, and derivation methods were described in detail to inform researchers and to facilitate new prospective cooperative studies.

Generation of cardiomyocytes from new human embryonic stem cell lines derived from poor-quality blastocysts.

It is shown that hES cell lines can be obtained from poor-quality blastocysts with the same efficiency as that obtained from good- or intermediate-quality Blastocysts.

Differences between human embryonic stem cell lines.

The evidence for differences between lines is reviewed, focusing on studies of pluripotency marker molecules, transcriptional profiling, genetic stability and epigenetic stability, for which there is most evidence.

The Use of Stem Cell-Derived Organoids in Disease Modeling: An Update

A synopsis of pluripotent stem cell-derived organoids and their use for disease modeling and other clinical applications is provided.

Derivation and Characterization of New hESC Lines from Supernumerary Embryos, Experience from Turkey

In this chapter, it was aimed to report the methods to derive 18 hESC lines which were established and characterized until the declaration of prohibition on h ESC research in Turkey by Health Ministry in 2005.

Factors Direct the Mesenchymal Stem Cell Lineage Commitment Reproductive Stem Cell Differentiation : Extracellular Matrix , Tissue Microenvironment , and Growth

The mesenchymal stem cells have awakened interest in regenerative medicine due to its high capability to proliferate and differentiate in multiple specialized lineages under defined conditions, and protocols of reproductive MSCs differentiation must be established.

In vitro culture of stem-like cells derived from somatic cell nuclear transfer bovine embryos of the Korean beef cattle species, HanWoo.

In conclusion, eSLCs from SCNT bovine embryos are generated using a 3i system that sustained stemness, normal karyotype and pluripotency, which was confirmed by in vitro and in vivo differentiation.

Reproductive Stem Cell Differentiation

The mesenchymal stem cells (MSCs) have awakened interest in regenerative medicine due to its high capability to proliferate and differentiate in multiple specialized lineages under defined conditions, and protocols of reproductive MSCs differentiation must be established.

Embryonic Stem Cells in Clinical Trials: Current Overview of Developments and Challenges.

An overview of clinical trial studies dealing with human embryonic stem cells and their advantages, limitations, and other specific concerns will be beneficial for understanding the standard and promising applications of cell and tissue-based therapeutic approaches and for developing novel therapeutic applications of hESC.



Multilineage Differentiation from Human Embryonic Stem Cell Lines

Because they have the dual ability to proliferate indefinitely and differentiate into multiple tissue types, human ES cells could potentially provide an unlimited supply of tissue for human transplantation.

Embryo-derived stem cells: of mice and men.

  • A. Smith
  • Biology
    Annual review of cell and developmental biology
  • 2001
The relationships between mouse embryonic stem cells, resident pluripotent cells in the embryo, and human embryo-derived cell lines are evaluated, and the prospects and challenges of embryo stem cell research are considered.

Embryonic stem cells: prospects for developmental biology and cell therapy.

This review focuses both on mouse and human ES cells with respect to in vitro propagation and differentiation as well as their use in basic cell and developmental biology and toxicology and presents prospects forhuman ES cells in tissue regeneration and transplantation.

Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro

The derivation of pluripotent embryonic stem (ES) cells from human blastocysts is described, providing a model to study early human embryology, an investigational tool for discovery of novel growth factors and medicines, and a potential source of cells for use in transplantation therapy.

Derivation, Characterization, and Differentiation of Human Embryonic Stem Cells

The derivation and characterization of six hES cell lines were presented and one cell line was clonally derived, which could be propagated in an undifferentiated pluripotent state.

Human Feeder Layers for Human Embryonic Stem Cells1

An additional animal-free culture system for hES cells is described, based on a feeder layer derived from foreskin and a serum-free medium, which enables proper analysis for foreign agents, genetic modification such as antibiotic resistance, and reduction of the enormous workload involved in the continuous preparation of new feeder lines.

BMP4 initiates human embryonic stem cell differentiation to trophoblast

The excitement and controversy surrounding the potential role of human embryonic stem (ES) cells in transplantation therapy have often overshadowed their potentially more important use as a basic

Stable genetic modification of human embryonic stem cells by lentiviral vectors.

Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells.

  • G. Martin
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1981
The establishment directly from normal preimplantation mouse embryos of a cell line that forms teratocarcinomas when injected into mice makes feasible the isolation of pluripotent cells lines from various types of noninbred embryo, including those carrying mutant genes.