Human embryonic stem cells: origin, properties and applications

@article{Semb2005HumanES,
  title={Human embryonic stem cells: origin, properties and applications},
  author={Henrik Semb},
  journal={APMIS},
  year={2005},
  volume={113}
}
  • H. Semb
  • Published 1 November 2005
  • Biology
  • APMIS
Human embryonic stem cells originate from the human preimplantation embryo. The derivation of the first human embryonic stem cells was reported in 1998. Since then we have learnt a great deal about how to isolate and culture these cells. Additionally, their stem cell phenotype and differentiation competence have been determined. Although it is expected that many basic biological properties, such as self‐renewal and cell specification, are evolutionary conserved, at least from the mouse, we lack… 
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References

SHOWING 1-10 OF 77 REFERENCES

Multilineage Differentiation from Human Embryonic Stem Cell Lines

TLDR
Because they have the dual ability to proliferate indefinitely and differentiate into multiple tissue types, human ES cells could potentially provide an unlimited supply of tissue for human transplantation.

Embryonic stem cells: prospects for developmental biology and cell therapy.

TLDR
This review focuses both on mouse and human ES cells with respect to in vitro propagation and differentiation as well as their use in basic cell and developmental biology and toxicology and presents prospects forhuman ES cells in tissue regeneration and transplantation.

Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro

TLDR
The derivation of pluripotent embryonic stem (ES) cells from human blastocysts is described, providing a model to study early human embryology, an investigational tool for discovery of novel growth factors and medicines, and a potential source of cells for use in transplantation therapy.

Derivation, Characterization, and Differentiation of Human Embryonic Stem Cells

TLDR
The derivation and characterization of six hES cell lines were presented and one cell line was clonally derived, which could be propagated in an undifferentiated pluripotent state.

Establishment of human embryonic stem cell-transfected clones carrying a marker for undifferentiated cells

Human Feeder Layers for Human Embryonic Stem Cells1

TLDR
An additional animal-free culture system for hES cells is described, based on a feeder layer derived from foreskin and a serum-free medium, which enables proper analysis for foreign agents, genetic modification such as antibiotic resistance, and reduction of the enormous workload involved in the continuous preparation of new feeder lines.

BMP4 initiates human embryonic stem cell differentiation to trophoblast

The excitement and controversy surrounding the potential role of human embryonic stem (ES) cells in transplantation therapy have often overshadowed their potentially more important use as a basic…

Stable genetic modification of human embryonic stem cells by lentiviral vectors.

Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells.

  • G. Martin
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1981
TLDR
The establishment directly from normal preimplantation mouse embryos of a cell line that forms teratocarcinomas when injected into mice makes feasible the isolation of pluripotent cells lines from various types of noninbred embryo, including those carrying mutant genes.

Human Adult Marrow Cells Support Prolonged Expansion of Human Embryonic Stem Cells in Culture

TLDR
The well‐studied hMSCs (and this animal cell‐ and serum‐free system) may provide a clinically and ethically feasible method to expand hES cells for novel cell therapies and this system may help to identify cytokines and adhesion molecules that are required for the self‐renewal of h ES cells.
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