Human embryonic stem cell lines derived from single blastomeres

@article{Klimanskaya2006HumanES,
  title={Human embryonic stem cell lines derived from single blastomeres},
  author={Irina V. Klimanskaya and Young Gie Chung and Sandy Becker and Shijiang Lu and Robert Lanza},
  journal={Nature},
  year={2006},
  volume={444},
  pages={481-485}
}
The derivation of human embryonic stem (hES) cells currently requires the destruction of ex utero embryos. A previous study in mice indicates that it might be possible to generate embryonic stem (ES) cells using a single-cell biopsy similar to that used in preimplantation genetic diagnosis (PGD), which does not interfere with the embryo’s developmental potential. By growing the single blastomere overnight, the resulting cells could be used for both genetic testing and stem cell derivation… 

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