Human embryonic stem cell lines derived from single blastomeres

  title={Human embryonic stem cell lines derived from single blastomeres},
  author={Irina V. Klimanskaya and Young Gie Chung and Sandy Becker and Shijiang Lu and Robert Lanza},
The derivation of human embryonic stem (hES) cells currently requires the destruction of ex utero embryos. A previous study in mice indicates that it might be possible to generate embryonic stem (ES) cells using a single-cell biopsy similar to that used in preimplantation genetic diagnosis (PGD), which does not interfere with the embryo’s developmental potential. By growing the single blastomere overnight, the resulting cells could be used for both genetic testing and stem cell derivation… 

Deriving bovine embryonic stem-like cells in defined conditions

Human embryonic stem cell lines generated without embryo destruction.

Challenges and approaches to the culture of pluripotent human embryonic stem cells.

This review describes how hESC lines have been derived from the inner cell mass of blastocysts or morula-stage embryos and the culture conditions used and discusses the challenges and future directions in optimization of standard culture conditions of hESCs for research purposes.

Gene-agnostic therapeutic approaches for inherited retinal degenerations

Key gene-agnostic approaches, including retinal cell reprogramming and replacement, neurotrophic support, immune modulation and optogenetics are reviewed, offering potential clinical benefits to all IRD patients.

I Am Not the Zygote I Came from because a Different Singleton Could Have Come from It

Many people believe that human beings begin to exist with the emergence of the 1-cell zygote at fertilization. I present a novel argument against this belief, one based on recently discovered facts

New Prospects for Retinal Pigment Epithelium Transplantation.

Current research involves development of better scaffolds, improved modulation of immune surveillance, and modification of the extracellular milieu to improve RPE survival and integration with host retina.

The Use of Stem Cell-Derived Organoids in Disease Modeling: An Update

A synopsis of pluripotent stem cell-derived organoids and their use for disease modeling and other clinical applications is provided.

Bacterial Lipase Neutralized Toxicity of Lipopolysaccharide on Chicken Embryo Cardiac Tissue

Bacterial lipase reduces detrimental effects of LPS on chicken embryo heart induced via Toll-like receptor signaling pathway, and total antioxidant capacity was increased in groups that received LPS’+’Lipase.



Manipulating the Mouse Embryo: A Laboratory Manual

This chapter discusses developmental Genetics and Embryology of the Mouse, including production of Transgenic and Chimeric Mice, and techniques for Visualizing Gene Products, Cells, Tissues, and Organ Systems.

An Atlas of Human Gametes and Conceptuses : An Illustrated Reference for Assisted Reproductive Technology

This review discusses the development of gamete maturation, spermatozoon formation, and the ability to Implant Abnormal Morphology of the Human Oocyte and Conceptus Cryopreservation.

Proliferation of blastomeres from biopsied cleavage stage human embryos in vitro: an alternative to blastocyst biopsy for preimplantation diagnosis.

Normally fertilized human embryos were biopsied at cleavage stages on the third day after in-vitro fertilization (IVF). One or two blastomeres at the 8-cell stage were removed and co-cultured with

Use of human gametes obtained from anonymous donors for the production of human embryonic stem cell lines.

Findings demonstrate that the future production of human embryonic stem cell lines for therapeutic use is possible with the use of donated gametes, and one of these cell lines has been shown to stain positive for alkaline phosphatase and stage-specific embryonic antigen (SSEA)-4 and negative for SSEA-1, express telomerase activity, and produce hCG when allowed to differentiate.

Developmental potential of single blastomeres of the rabbit egg.

Tarkowski & Wroblewska (1967) have recently presented a detailed account of the development of isolated blasto¬ meres of fourand eight-cell mouse eggs maintained under in vitro conditions for 36 to 48 hr.

Cdx2 Gene Expression and Trophectoderm Lineage Specification in Mouse Embryos

It is shown that the transcription factor Cdx2 is expressed in the nuclei of cells derived from the late-d dividing but not the first-dividing blastomere of two-cell embryos and, by lineage tracing and RNA interference knock-down experiments, that this lagging cell is the precursor of trophectoderm.

Fast and reliable sexing of prosimian and human DNA

A single, conserved primer pair is designed that is useful for fast and reliable molecular sexing of prosimian primates and was successful in seven species of lemurs, as well as humans.