Human embryonic stem cell-derived cells rescue visual function in dystrophic RCS rats.
@article{Lund2006HumanES, title={Human embryonic stem cell-derived cells rescue visual function in dystrophic RCS rats.}, author={Raymond D. Lund and Shaomei Wang and Irina V. Klimanskaya and Toby M. Holmes and Rebeca Ramos-Kelsey and Bin Lu and S. V. Girman and Nicholas Bischoff and Yves Sauve and Robert Lanza}, journal={Cloning and stem cells}, year={2006}, volume={8 3}, pages={ 189-99 } }
Embryonic stem cells promise to provide a well-characterized and reproducible source of replacement tissue for human clinical studies. An early potential application of this technology is the use of retinal pigment epithelium (RPE) for the treatment of retinal degenerative diseases such as macular degeneration. Here we show the reproducible generation of RPE (67 passageable cultures established from 18 different hES cell lines); batches of RPE derived from NIH-approved hES cells (H9) were…
411 Citations
Protective Effects of Human iPS-Derived Retinal Pigment Epithelium Cell Transplantation in the Retinal Dystrophic Rat
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It is demonstrated that iPS cells can be differentiated into functional iPS-RPE and that transplantation of these cells can facilitate the short-term maintenance of photoreceptors through phagocytosis ofPhotoreceptor outer segments.
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- Biology, MedicineStem cells
- 2007
Since the umbilical tissue‐derived cells demonstrated the best photoreceptor rescue and, unlike mesenchymal stem cells, were capable of sustained population doublings without karyotypic changes, it is proposed that they may provide utility as a cell source for the treatment of retinal degenerative diseases such as retinitis pigmentosa.
Human embryonic stem cells for retinal repair : preclinical in vitro and in vivo studies for the treatment of age-related macular degeneration with human embryonic stem cell-derived retinal pigment epithelial cells
- Biology, Medicine
- 2018
Evidence is shown that allogeneic replacement therapy using subretinal injection of hESC-RPE in suspension can be a successful treatment if (i) the derived cells retain native RPE cell properties; (ii) the cells are transplanted early enough so theSubretinal milieu supports their integration; and (iii) the Cells can be engineered so that they can evade the host immune system and consequent graft rejection.
Differentiation of Human Embryonic Stem Cells and Human Induced Pluripotent Stem Cells into Retinal Pigment Epithelium
- Biology, Medicine
- 2012
Improvements are needed to increase the yield and purity of the RPE cultures in order to reach sufficient amount of maturated cells with RPE characteristics, which can be utilized safely in therapeutic use.
Enhanced functional integration of human photoreceptor precursors into human and rodent retina in an ex vivo retinal explant model system.
- BiologyTissue engineering. Part A
- 2015
The utility of an ex vivo model system to define factors, such as TSP-1, which could influence integration efficiency in future in vivo experiments in models of retinal degeneration are demonstrated.
Directed differentiation of human embryonic stem cells into functional retinal pigment epithelium cells.
- Biology, MedicineCell stem cell
- 2009
RPE and Stem Cell Therapy
- Biology, Medicine
- 2020
Two RPE cell transplantation strategies—each with their own pros and cons—are currently under development: injection of a single-cell suspension, and transplantation of intact RPE sheet with or without a biomaterial-based scaffold.
Utilizing stem cell-derived RPE cells as a therapeutic intervention for age-related macular degeneration.
- Biology, MedicineAdvances in experimental medicine and biology
- 2014
Work reported by several groups, including the own, that clearly demonstrate that transplanted RPE cells can provide anatomical and functional photoreceptor rescue in animal models of retinal degeneration are summarized.
Transplantation of Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium for the Treatment of Macular Degeneration
- Medicine, Biology
- 2015
In the first ever clinical trial of subretinal hESC-RPE transplantation in humans, no adverse events related to the treatment, such as hyperproliferation, tumorigenicity, inflammation, infection, or rejection, were noted in either patient.
Transplantation of GMP-grade human iPSC-derived retinal pigment epithelial cells in rodent model: the first pre-clinical study for safety and efficacy in China.
- Biology, MedicineAnnals of translational medicine
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The transplanted iRPE cells survived for at least 19 weeks and maintained visual function for 15 weeks, and are considered a promising source of cell replacement therapy for AMD.
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