Human dihydrofolate reductase influences the sensitivity of the malaria parasite Plasmodium falciparum to ketotifen – A cautionary tale in screening transgenic parasites

@inproceedings{Tran2016HumanDR,
  title={Human dihydrofolate reductase influences the sensitivity of the malaria parasite Plasmodium falciparum to ketotifen – A cautionary tale in screening transgenic parasites},
  author={Phuong N. Tran and Cameron J. Tate and Melanie C. Ridgway and Kevin J. Saliba and Kiaran Kirk and Alexander G Maier},
  booktitle={International journal for parasitology. Drugs and drug resistance},
  year={2016}
}
Ketotifen has recently been reported to inhibit the growth of both asexual and sexual malaria parasites. A parasite transporter, PfgABCG2, has been implicated in its mechanism of action. Human dihydrofolate reductase (hDHFR) is the most commonly used selectable marker to create transgenic Plasmodium falciparum cell lines. Growth assays using transgenic P… CONTINUE READING