Human defensin 5 disulfide array mutants: disulfide bond deletion attenuates antibacterial activity against Staphylococcus aureus.

@article{Wanniarachchi2011HumanD5,
  title={Human defensin 5 disulfide array mutants: disulfide bond deletion attenuates antibacterial activity against Staphylococcus aureus.},
  author={Yoshitha A. Wanniarachchi and Piotr Kaczmarek and Andrea Wan and Elizabeth M Nolan},
  journal={Biochemistry},
  year={2011},
  volume={50 37},
  pages={8005-17}
}
Human α-defensin 5 (HD5, HD5(ox) to specify the oxidized and disulfide linked form) is a 32-residue cysteine-rich host-defense peptide, expressed and released by small intestinal Paneth cells, that exhibits antibacterial activity against a number of Gram-negative and -positive bacterial strains. To ascertain the contributions of its disulfide array to structure, antimicrobial activity, and proteolytic stability, a series of HD5 double mutant peptides where pairs of cysteine residues… CONTINUE READING