Human cytomegalovirus maturational proteinase: expression in Escherichia coli, purification, and enzymatic characterization by using peptide substrate mimics of natural cleavage sites.

Abstract

The proteolytic processing of the human cytomegalovirus (HCMV) assembly protein, resulting in truncation of its C terminus, is an essential step in virion maturation. The proteinase responsible for this cleavage is the amino-terminal half of the protein encoded by the UL80a open reading fame. We have obtained high expression levels of this 256-amino-acid… (More)

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@article{Burck1994HumanCM, title={Human cytomegalovirus maturational proteinase: expression in Escherichia coli, purification, and enzymatic characterization by using peptide substrate mimics of natural cleavage sites.}, author={P J Burck and Daniela Berg and T-S. Luk and L M Sassmannshausen and Mark Wakulchik and D. Paul Smith and Hansen M. Hsiung and Guillaume Becker and W. P. Gibson and Elcira Villarreal}, journal={Journal of virology}, year={1994}, volume={68 5}, pages={2937-46} }