Human cytidine triphosphate synthetase 1 interacting proteins.

  title={Human cytidine triphosphate synthetase 1 interacting proteins.},
  author={Matthew J Higgins and David L Loiselle and Timothy A. J. Haystead and Lee M Graves},
  journal={Nucleosides, nucleotides & nucleic acids},
  volume={27 6},
We investigated the interacting proteins and intracellular localization of CTP synthetase 1 (CTPS1) in mammalian cells. CTPS1 interacted with a GST- peptidyl prolyl isomerase, Pin1 fusion (GST-Pin1) in a Ser 575 (S575) phosphorylation-dependent manner. Immunoprecipitation experiments demonstrated that CTPS1 also bound tubulin, and thirteen additional coimmunoprecipitating proteins were identified by mass spectrometry. Immunolocalization experiments showed that tubulin and CTPS1 colocalized… CONTINUE READING