Human complement component C4. Structural studies on the fragments derived from C4b by cleavage with C3b inactivator.

@article{Press1981HumanCC,
  title={Human complement component C4. Structural studies on the fragments derived from C4b by cleavage with C3b inactivator.},
  author={E M. Press and J M Gagnon},
  journal={The Biochemical journal},
  year={1981},
  volume={199 2},
  pages={351-7}
}
1. One of the activation products of C4, C4b, was prepared, and the reactive thiol group on the alpha'-chain was radioactively labelled with iodo[2-14C]acetic acid. The alpha'-chain was isolated and the N-terminal amino acid sequence of the first 13 residues was determined. 2. C4b was cleaved by C3bINA in the presence of C4b-binding protein and C4d and C4c isolated. The radioactive label and therefore the reactive thiol group were located to C4d. 3. C4c was reduced and alkylated and the two… CONTINUE READING