Human colonic crypts in culture: segregation of immunochemical markers in normal versus adenoma-derived

@article{Dame2014HumanCC,
  title={Human colonic crypts in culture: segregation of immunochemical markers in normal versus adenoma-derived},
  author={Michael K Dame and Yan Jiang and Henry D. Appelman and Kelly D Copley and Shannon D. McClintock and Muhammad Nadeem Aslam and Durga Attili and B Joseph Elmunzer and Dean E. Brenner and James Varani and Dr D. Kim Turgeon},
  journal={Laboratory Investigation},
  year={2014},
  volume={94},
  pages={222-234}
}
In order to advance a culture model of human colonic neoplasia, we developed methods for the isolation and in vitro maintenance of intact colonic crypts from normal human colon tissue and adenomas. Crypts were maintained in three-dimensional Matrigel culture with a simple, serum-free, low Ca2+ (0.15 mM) medium. Intact colonic crypts from normal human mucosa were viably maintained for 3–5 days with preservation of the in situ crypt-like architecture, presenting a distinct base and apex. Abnormal… CONTINUE READING
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