Human carcinoembryonic antigen cDNA expressed in rat carcinoma cells can function as target antigen for tumor localization of antibodies in nude rats and as rejection antigen in syngeneic rats.

@article{Pelegrin1992HumanCA,
  title={Human carcinoembryonic antigen cDNA expressed in rat carcinoma cells can function as target antigen for tumor localization of antibodies in nude rats and as rejection antigen in syngeneic rats.},
  author={Andr{\'e} Pelegrin and Alexey Terskikh and Daniel Hayoz and Y Chalandon and Niels Olivier Olsson and S Folli and Franz Buchegger and B Kromer and Katrin Schwarz and Monique Martin},
  journal={International journal of cancer},
  year={1992},
  volume={52 1},
  pages={110-9}
}
We have tried to develop a new model consisting of rats transplanted with syngeneic colon carcinoma PROb cells transfected with cDNA coding for the carcinoembryonic antigen (CEA), the human tumor marker most commonly used as target for MAbs. The antigenic density of the 4 CEA-expressing clones selected for a precise characterization ranged from 5 x 10(4) to 1 x 10(6) CEA molecules per cell. In all clones the CEA was shown to be attached to the membrane by a phosphatidylinositol (PI) anchor… CONTINUE READING