Human breast carcinoma cells cultured in the presence of serum shed membrane vesicles rich in gelatinolytic activities.

@article{Dolo1994HumanBC,
  title={Human breast carcinoma cells cultured in the presence of serum shed membrane vesicles rich in gelatinolytic activities.},
  author={Vincenza Dolo and Angela Ginestra and Guilio Ghersi and Hideaki Nagase and Maria Letizia Vittorelli},
  journal={Journal of submicroscopic cytology and pathology},
  year={1994},
  volume={26 2},
  pages={173-80}
}
Human breast carcinoma cell lines, 8701-BC and MCF-7, in culture shed membrane vesicles with similar morphology. Vesicles shed in the presence of serum were rich in gelatinolytic activities, but not those obtained in the absence of serum. Zymographic analyses of the vesicles from 8701-BC and MCF-7, using gelatin as substrate, showed three predominant activities at 68-kDa, 97-kDa, and above 200-kDa. The ratio of the three activities was similar in the vesicles recovered from the two cell lines… CONTINUE READING