Human brain fibroblast growth factor. Isolation and partial chemical characterization.

@article{Bhlen1985HumanBF,
  title={Human brain fibroblast growth factor. Isolation and partial chemical characterization.},
  author={Peter B{\"o}hlen and Fred S. Esch and Andrew Baird and Kent L. Jones and Denis Gospodarowicz},
  journal={FEBS letters},
  year={1985},
  volume={185 1},
  pages={177-81}
}
Fibroblast growth factor (FGF) has been purified to homogeneity from human brain by a procedure involving salt precipitation, cation-exchange chromatography, Heparin-Sepharose affinity chromatography and reverse-phase HPLC. Isolation was monitored by radioimmunoassay and/or by testing column fractions for their capacity to stimulate the proliferation of vascular endothelial cells in vitro. The amino-terminal sequence of human brain FGF was determined as Pro-Ala-Leu-Pro-Glu-Asp-Gly-Gly-Ser-Gly… CONTINUE READING

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