Human betaine-homocysteine methyltransferase is a zinc metalloenzyme.

  title={Human betaine-homocysteine methyltransferase is a zinc metalloenzyme.},
  author={Norman S Millian and Timothy A. Garrow},
  journal={Archives of biochemistry and biophysics},
  volume={356 1},
We have overexpressed recombinant human liver betaine-homocysteine methyltransferase (BHMT; EC in Escherichia coli and have purified the enzyme to homogeneity. The Michaelis constants for betaine and l-homocysteine are 2.2 mM and 4 microM, respectively. Analysis of the pure protein for metals by inductively coupled plasma emission spectrometry indicate that the recombinant enzyme contains zinc. Extensive dialysis in buffer containing high levels of EDTA could not strip the protein of… CONTINUE READING
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