Human beta-defensin-3 producing cells in septic implant loosening

  title={Human beta-defensin-3 producing cells in septic implant loosening},
  author={Jaakko Lev{\'o}n and Ahmed Al-Samadi and Zygmunt Mackiewicz and Andrej Coer and Rihard Treb{\vs}e and Eero Waris and Yrj{\"o} T. Konttinen},
  journal={Journal of Materials Science: Materials in Medicine},
Human β-defensin-3 (hBD-3) has been found in synovial fluid and later in periprosthetic tissues in septic joint implant loosening. The aim of the present study was to identify its cellular sources. Tissue samples from 12 patients were analyzed. A fully automatic Leica BOND MAX staining robot was used. Affinity-purified rabbit anti-human hBD-3 IgG was applied in a two-layer horse radish peroxidase/anti-rabbit-labeled polymer method. Double immunofluorescence of hBD3 together with CD68, CD31… CONTINUE READING