Human beta-cell glucokinase. Dual role of Ser-151 in catalysis and hexose affinity.

@article{Xu1995HumanBG,
  title={Human beta-cell glucokinase. Dual role of Ser-151 in catalysis and hexose affinity.},
  author={L. Z. Xu and Robert W. Harrison and Irene T. Weber and S. J. Pilkis},
  journal={The Journal of biological chemistry},
  year={1995},
  volume={270 17},
  pages={9939-46}
}
Glucokinase is distinguished from yeast hexokinase and low Km mammalian hexokinases by its low affinity for glucose and its cooperative behavior, even though glucose binding residues and catalytic residues are highly conserved in all of these forms of hexokinase. The roles of Ser-151 and Asn-166 as determinants of hexose affinity and cooperative behavior of human glucokinase have been evaluated by site-directed mutagenesis, expression and purification of the wild-type and mutant enzymes, and… CONTINUE READING

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