Human basophil degranulation triggered by very dilute antiserum against IgE

@article{Davenas1988HumanBD,
  title={Human basophil degranulation triggered by very dilute antiserum against IgE},
  author={Elisabeth Davenas and Francis Beauvais and J. Amara and Menachem Oberbaum and Boaz Robinzon and A. Miadonnai and Alberto Tedeschi and Bruce H. Pomeranz and P. Fortner and Philippe Belon and J. L. Sainte-Laudy and Bernard Poitevin and Jacques E. Benveniste},
  journal={Nature},
  year={1988},
  volume={333},
  pages={816-818}
}
When human polymorphonuclear basophils, a type of white blood cell with antibodies of the immunoglobulin E (IgE) type on its surface, are exposed to anti-IgE antibodies, they release histamine from their intracellular granules and change their staining properties. The latter can be demonstrated at dilutions of anti-IgE that range from 1 x 10(2) to 1 x 10(120); over that range, there are successive peaks of degranulation from 40 to 60% of the basophils, despite the calculated absence of any anti… 
Human basophil degranulation is not triggered by very dilute antiserum against human IgE
TLDR
The findings of Benveniste and co-workers, who reported in 1988 that degranulation of human basophil leukocytes is triggered by very dilute antiserum against IgE, are reproduced.
Inhibition of human basophil activation by high dilutions of histamine
TLDR
The inhibition of anti-IgE induced human basophil activation by histamine dilutions ranging from 10−10M to 10−38M, the most frequent inhibition observed being for 10−18, 10−20, and 10−32M, in agreement with the previous results.
Inhibition of CD203c membrane up-regulation in human basophils by high dilutions of histamine: a controlled replication study
TLDR
It is shown that low and high dilutions of histamine inhibit CD203c up-regulation in anti-IgE stimulated basophils, using a strictly standardized flow cytometry protocol and a new dilution/succussion procedure.
Analysis of immunosuppressive activity of serial dilutions of histamine on human basophil activation by flow cytometry
TLDR
This study investigated whether it could detect modulation of anti-IgE induced basophil activation by histamine with oUf flow cytometric method and found effects by counting unactivated cells.
Mechanical agitation of very dilute antiserum against IgE has no effect on basophil staining properties
TLDR
The effect of extreme dilutions of anti-IgE, reported by Davenas et al.2, needs further clarification and that in this process the reproducibility of results between experimenters should be carefully determined.
Sensitive Flow Cytometric Method to Test Basophil Activation Influenced by Homeopathic Histamine Dilutions
TLDR
A 3-color flow cytometric method with enhanced accuracy was established to measure the effects from homeopathic solutions in an in-vitro cell system and documented an unequivocally inhibited basophil activation by anti-IgE.
Application of flow cytometry to the analysis of the immunosuppressive effect of histamine dilutions on human basophil activation: Effect of cimetidine
TLDR
The aim of this study was to reinvestigate the ability of cimetidine to reverse the immunomodulatory effects of histamine using the flow cytometric method and confirm the biological activity of ultra low doses of histamines.
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TLDR
This very simple test explores the allergic sensitivity at the cellular level, probably through IgE‐dependent sensilization of the basophil, and positively correlates with histamine release and radioallergosorbent test (RAST).
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The investigation of the clinical efficacy of high dilutions of Lung his and Apis mel should be envisaged in allergic diseases in parallel with in vitro and ex vivo biological assays.
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TLDR
It is concluded that the modified BDT provides a worthwhile addition to the in vitro testing of immediate‐type hypersensitivity states.
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