Human basophil degranulation triggered by very dilute antiserum against IgE
@article{Davenas1988HumanBD, title={Human basophil degranulation triggered by very dilute antiserum against IgE}, author={Elisabeth Davenas and Francis Beauvais and J. Amara and Menachem Oberbaum and Boaz Robinzon and A. Miadonnai and Alberto Tedeschi and Bruce H. Pomeranz and P. Fortner and Philippe Belon and J. L. Sainte-Laudy and Bernard Poitevin and Jacques E. Benveniste}, journal={Nature}, year={1988}, volume={333}, pages={816-818} }
When human polymorphonuclear basophils, a type of white blood cell with antibodies of the immunoglobulin E (IgE) type on its surface, are exposed to anti-IgE antibodies, they release histamine from their intracellular granules and change their staining properties. The latter can be demonstrated at dilutions of anti-IgE that range from 1 x 10(2) to 1 x 10(120); over that range, there are successive peaks of degranulation from 40 to 60% of the basophils, despite the calculated absence of any anti…
586 Citations
Human basophil degranulation is not triggered by very dilute antiserum against human IgE
- BiologyNature
- 1993
The findings of Benveniste and co-workers, who reported in 1988 that degranulation of human basophil leukocytes is triggered by very dilute antiserum against IgE, are reproduced.
Inhibition of human basophil activation by high dilutions of histamine
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The inhibition of anti-IgE induced human basophil activation by histamine dilutions ranging from 10−10M to 10−38M, the most frequent inhibition observed being for 10−18, 10−20, and 10−32M, in agreement with the previous results.
Inhibition of CD203c membrane up-regulation in human basophils by high dilutions of histamine: a controlled replication study
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It is shown that low and high dilutions of histamine inhibit CD203c up-regulation in anti-IgE stimulated basophils, using a strictly standardized flow cytometry protocol and a new dilution/succussion procedure.
Analysis of immunosuppressive activity of serial dilutions of histamine on human basophil activation by flow cytometry
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This study investigated whether it could detect modulation of anti-IgE induced basophil activation by histamine with oUf flow cytometric method and found effects by counting unactivated cells.
Replication study concerning the effects of homeopathic dilutions of histamine on human basophil degranulation in vitro.
- Medicine, BiologyComplementary therapies in medicine
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Mechanical agitation of very dilute antiserum against IgE has no effect on basophil staining properties
- Biology, MedicineExperientia
- 2005
The effect of extreme dilutions of anti-IgE, reported by Davenas et al.2, needs further clarification and that in this process the reproducibility of results between experimenters should be carefully determined.
Sensitive Flow Cytometric Method to Test Basophil Activation Influenced by Homeopathic Histamine Dilutions
- Biology, MedicineComplementary Medicine Research
- 2003
A 3-color flow cytometric method with enhanced accuracy was established to measure the effects from homeopathic solutions in an in-vitro cell system and documented an unequivocally inhibited basophil activation by anti-IgE.
Improvement of flow cytometric analysis of basophil activation inhibition by high histamine dilutions. A novel basophil specific marker: CD 203c.
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Application of flow cytometry to the analysis of the immunosuppressive effect of histamine dilutions on human basophil activation: Effect of cimetidine
- BiologyInflammation Research
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The aim of this study was to reinvestigate the ability of cimetidine to reverse the immunomodulatory effects of histamine using the flow cytometric method and confirm the biological activity of ultra low doses of histamines.
Inhibition of basophil activation by histamine: a sensitive and reproducible model for the study of the biological activity of high dilutions.
- BiologyHomeopathy : the journal of the Faculty of Homeopathy
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References
SHOWING 1-10 OF 14 REFERENCES
The human basophil degranulation test as an invitro method for the diagnosis of allergies
- Medicine, BiologyClinical allergy
- 1981
This very simple test explores the allergic sensitivity at the cellular level, probably through IgE‐dependent sensilization of the basophil, and positively correlates with histamine release and radioallergosorbent test (RAST).
In vitro immunological degranulation of human basophils is modulated by lung histamine and Apis mellifica.
- Biology, MedicineBritish journal of clinical pharmacology
- 1988
The investigation of the clinical efficacy of high dilutions of Lung his and Apis mel should be envisaged in allergic diseases in parallel with in vitro and ex vivo biological assays.
A new concept of triggering mechanisms of IgE-mediated histamine release.
- Biology, ChemistryMonographs in allergy
- 1979
The receptor with high affinity for immunoglobulin E.
- BiologyAnnual review of immunology
- 1986
The Fc receptor with high affinity for monomeric immunoglobulin E (IgE}-is only one of several proteins that interact with IgE (1-5), and its special characteristic is its distribution on the plasma membrane of mast cells and basophils exclusively.
Release of platelet-activating factor in human pathology. I. Evidence for the occurrence of basophil degranulation and release of platelet-activating factor in systemic lupus erythematosus.
- Biology, MedicineLaboratory investigation; a journal of technical methods and pathology
- 1981
The concomitance of basophil degranulation and release of PAF-acether, together with the morphologic evidence of the interaction between degranulating basophils and aggregated platelets, was strongly suggestive of release ofPAF-ACether from basophilia in systemic lupus erythematosus.
Evaluation of the human basophil degranulation test using the commercially available Baso‐kit as a test of immediate‐type hypersensitivity in hay‐fever sufferers
- MedicineClinical allergy
- 1984
It is concluded that the modified BDT provides a worthwhile addition to the in vitro testing of immediate‐type hypersensitivity states.
Effect of mouse peritoneal macrophages of orally administered very high dilutions of silica.
- BiologyEuropean journal of pharmacology
- 1987
IS HOMOEOPATHY A PLACEBO RESPONSE? CONTROLLED TRIAL OF HOMOEOPATHIC POTENCY, WITH POLLEN IN HAYFEVER AS MODEL
- Medicine, PsychologyThe Lancet
- 1986