Human basophil degranulation is not triggered by very dilute antiserum against human IgE

  title={Human basophil degranulation is not triggered by very dilute antiserum against human IgE},
  author={S. J. Hirst and N. Hayes and J. Burridge and F. Pearce and J. Foreman},
We have attempted to reproduce the findings of Benveniste and co-workers, who reported in 1988 that degranulation of human basophil leukocytes is triggered by very dilute (102–10120) antiserum against IgE. The results were contrary to conventional scientific theory and were not satisfactorily explained. Following as closely as possible the methods of the original study, we can find no evidence for any periodic or polynomial change of degranulation as a function of anti-lgE dilution. Our results… Expand
Replication study concerning the effects of homeopathic dilutions of histamine on human basophil degranulation in vitro.
Histamine at the dilutions 10(-2)M and 10(-22)M was associated with a significant inhibition of basophil degranulation, but the previously reported large effects of homeopathic histamine dilutions on basophils function of the examined donor were not confirmed. Expand
Inhibition of basophil activation by histamine: a sensitive and reproducible model for the study of the biological activity of high dilutions.
Successively, using different models to study of human and murine basophil activation, it is demonstrated that high dilutions of histamine, in the range of 15-17CH induce a reproducible biological effect. Expand
Basophil models of homeopathy: a sceptical view.
  • M. Ennis
  • Medicine
  • Homeopathy : the journal of the Faculty of Homeopathy
  • 2010
It is recommended that a multi-centre trial be performed to hopefully put an end to this "never-ending story" of trying to find out if high dilutions of histamine have a negative feedback effect on the activation of basophils by anti-IgE. Expand
Inhibition of CD203c membrane up-regulation in human basophils by high dilutions of histamine: a controlled replication study
It is shown that low and high dilutions of histamine inhibit CD203c up-regulation in anti-IgE stimulated basophils, using a strictly standardized flow cytometry protocol and a new dilution/succussion procedure. Expand
Improvement of flow cytometric analysis of basophil activation inhibition by high histamine dilutions. A novel basophil specific marker: CD 203c.
These new flow cytometric protocols confirmed that high dilutions of histamine may inhibit basophil activation and that the inhibitory effect is not restricted to IgE-dependent activation. Expand
Histamine dilutions modulate basophil activation
In 3 different types of experiment, it has been shown that high dilutions of histamine may indeed exert an effect on basophil activity, with the greatest inhibitory effects seen at lower levels of stimulation. Expand
Original Research Papers Histamine dilutions modulate basophil activation
Background: In order to demonstrate that high dilutions of histamine are able to inhibit basophil activation in a reproducible fashion, several techniques were used in different researchExpand
Sensitive Flow Cytometric Method to Test Basophil Activation Influenced by Homeopathic Histamine Dilutions
A 3-color flow cytometric method with enhanced accuracy was established to measure the effects from homeopathic solutions in an in-vitro cell system and documented an unequivocally inhibited basophil activation by anti-IgE. Expand
Survey of immuno-allergological ultra high dilution research.
  • B. Poitevin
  • Medicine
  • Homeopathy : the journal of the Faculty of Homeopathy
  • 2015
Despite considerable research activity in immuno-allergology and a great increase in the number of publications, there is still not in this domain a "gold standard" trial in basic research in homeopathy. Expand
Immunology and Homeopathy. 2. Cells of the Immune System and Inflammation
This review of literature unknown to most people will give an original and useful insight into the ‘state-of-the-art’ of homeopathy, without final conclusions ‘for’ or ‘against’ this modality. Expand


Human basophil degranulation triggered by very dilute antiserum against IgE
When human polymorphonuclear basophils are exposed to anti-IgE antibodies, they release histamine from their intracellular granules and change their staining properties, and transmission of the biological information could be related to the molecular organization of water. Expand