Human astrovirus diagnosis and typing: current and future prospects

@article{Guix2005HumanAD,
  title={Human astrovirus diagnosis and typing: current and future prospects},
  author={Susana Guix and Albert Bosch and Rosa M. Pint{\'o}},
  journal={Letters in Applied Microbiology},
  year={2005},
  volume={41}
}
Human astroviruses (HAstV) are important human pathogens causing gastroenteritis worldwide. The increased recognition of astroviruses as the cause of sporadic outbreaks of disease is due to the recent availability of improved diagnostic methods. During the last decade, most epidemiological surveys have chosen astrovirus‐specific RT‐PCR as screening methods. In addition to serotyping by molecular techniques, new typing methods are being developed that may also identify other viral properties… 

Astrovirus Diagnostics

This work reviews the past and current methods for astrovirus detection and their uses in both research laboratories and for medical diagnostic purposes.

Novel coronaviruses and astroviruses in bats

The discoveries of these novel bats viruses not only suggested that bats are important hosts for these virus families, but also reiterated the role of bats as a reservoir of viruses that might pose a zoonotic threat to human health.

Genomic Analysis of Closely Related Astroviruses

The analysis of the capsid gene demonstrated evidence for both site-specific positive selection and purifying selection, and suggests that the TAstV species contains multiple subtypes and that recombination events have occurred across the astrovirus genome.

Serological Studies Confirm the Novel Astrovirus HMOAstV-C as a Highly Prevalent Human Infectious Agent

This work characterized human anti-IgG antibody responses by luciferase immunoprecipitation systems (LIPS) against two protein fragments derived from the capsid protein of the novel HMOAstV-C astrovirus and validated LIPS as a facile and effective approach for identifying humoral responses to novel infectious agents.

Molecular epidemiology and genetic diversity of human astrovirus in South Korea from 2002 to 2007.

  • A. JeongH. Jeong D. Cheon
  • Biology
    Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
  • 2011
The findings indicate that HAstV is less common but, even so, a potentially important viral agent of gastroenteritis in South Korea, with significant genetic diversity among circulatingHAstV strains.

Human Astroviruses

This review focuses on the basic virology, pathogenesis, host response, epidemiology, diagnostic assays, and prevention strategies for HAstVs.

Detection and molecular characterization of Porcine astrovirus strains associated with swine diarrhea

Porcine astrovirus type 4 (PAstV-4) is present on swine farms in the United States and that it may be associated with diarrhea either alone or in combination with other enteric viruses.

New astrovirus in human feces from Burkina Faso.

  • T. PhanJ. Nordgren E. Delwart
  • Biology, Medicine
    Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
  • 2014

Human Astrovirus Gastroenteritis in Children, Madagascar, 2004–2005

In a 13-month study, 5 HAstV isolates were detected in fecal samples from 237 children by reverse transcription–PCR, andylogenetic analysis showed the cocirculation of usual and unusual HAstVs.
...

References

SHOWING 1-10 OF 29 REFERENCES

Molecular characterization and sequence analysis of human astroviruses circulating in Hungary.

Molecular epidemiology of astrovirus infection in Italian children with gastroenteritis.

The results indicated that HAstV should be considered as a potential diarrhoeal pathogen in Italian children.

Astrovirus infection in children

Astroviruses are increasingly recognized as significant gastrointestinal pathogens and the understanding of molecular epidemiology and molecular processing of the virus may lead to specific prevention strategies.

The changing epidemiology of astrovirus-associated gastroenteritis: a review.

In the absence of obvious strategies to prevent astrovirus-associated diarrhea, vaccines might be considered if further studies establish that the disease burden would render such a vaccine cost-effective.

Typing of human astroviruses from clinical isolates by enzyme immunoassay and nucleotide sequencing

A typing enzyme immunoassay (TYPE-EIA) was used to determine the antigenic types of 64 astrovirus-positive specimens from nine collections from seven countries, finding most strains of the same type were identical.

Detection and genetic differentiation of human astroviruses: phylogenetic grouping varies by coding region

A reverse transcription-polymerase chain reaction assay using primers targeted to a nonstructural protein coding region that allowed sensitive detection and genetic typing of representative strains of seven astrovirus serotypes indicated two distinct genogroups of sequences in open reading frame 1a (ORF 1a).

Persistent gastroenteritis in children infected with astrovirus: Association with serotype‐3 strains

The relationship between cases of persistent diarrhoea and the levels and type of human astrovirus was investigated and quantifying astroviruses in faecal samples by a competitive RT‐PCR showed a threshold of positivity of 3.4 × 104 genomes per gram of faeces.

Molecular epidemiology of human astrovirus diarrhea among children from a periurban community of Mexico City.

RT-PCR and sequence analysis provided more detailed epidemiology of HAstV in the community than did antigenic detection methods.

Molecular epidemiology of childhood astrovirus infection in child care centers.

Astrovirus was a significant cause of diarrhea outbreaks, and 2 antigenic types were present in the community during 1 diarrhea season.

Detection of Astroviruses from Stool Samples in Japan Using Reverse Transcription and Polymerase Chain Reaction Amplification

The RT‐PCR method was useful for detecting astroviruses from clinical samples, especially serotypes 1 and 2, the most predominant serotypes in the world.