Human antisera detect a Plasmodium falciparum genomic clone encoding a nonapeptide repeat

  title={Human antisera detect a Plasmodium falciparum genomic clone encoding a nonapeptide repeat},
  author={Michael Koenen and Artur Scherf and Odile Mercereau and Gordon Langsley and L Sibilli and Philippe Dubois and Luis Pereira da Silva and Benno M{\"u}ller-Hill},
Plasmodium falciparum causes malaria infections in its human host. Its wide distribution in tropical countries is a major world health problem. Before a vaccine can be produced, the identification and characterization of parasite antigens is necessary. This can be achieved by the cloning and subsequent analysis of genes coding for parasite antigens1–4. Recently established cDNA banks allow the expression of cDNA derived from the simian parasite Plasmodium knowlesi5 and P. falciparum6,7 in… 

Cross–reactive antigenic determinants present on different Plasmodium falciparum blood–stage antigens

Summary A gene encoding a previously undescribed antigen of Plasmodium falciparum has been isolated from a genomic expression library by use of a pool of human immune sera. Northern blot analysis

Isolation of the gene for a glycophorin-binding protein implicated in erythrocyte invasion by a malaria parasite.

The characterization of the Plasmodium falciparum glycophorin-binding protein represents one approach toward designing serologic agents to block the parasite's development in the vertebrate host.

Polymorphic antigens in Plasmodium falciparum

The structural basis of diversity in these and other Pfalciparum antigens is described in this review and reveals a remarkable degree of diversity among allelic genes for several different asexual blood stage antIGens.

Pfs2400 can mediate antibody-dependent malaria transmission inhibition and may be the Plasmodium falciparum 11.1 gene product

Monoclonal antibodies (mAb) have been raised against Plasmodium falciparum gametocyte stage protein extracts, in an effort to identify novel parasite antigens that might mediate malaria transmission-

Antigenic repeat structures in proteins of Plasmodium falciparum.

Synthetic peptides have been used to show that natural antibody responses to a strain-specific Plasmodium falciparum S antigen are largely directed against epitopes encoded in an 11-amino acid sequence that is repeated approximately 100 times in the molecule.

Size variation in chromosomes from independent cultured isolates of Plasmodium falciparum

Fractionation by PFG electrophoresis of chromosomal DNA molecules from P. falciparum is described into at least seven discrete species which vary in size by up to 20% between different isolates, and two of the chromosomes seem to contain the same sequences.



Expression of Plasmodium falciparum blood-stage antigens in Escherichia coli: detection with antibodies from immune humans.

An expression library of P. falciparum cDNA sequences, cloned in Escherichia coli, which should facilitate new approaches to the identification of potential vaccine molecules.

Target antigens of purified human immunoglobulins which inhibit growth of Plasmodium falciparum in vitro

Sera from clinically defined patient groups and functionally defined immunoglobulin may be used as probes to identify the relevant parasite antigens involved in host protection (‘host-protective antigen’).

Antigenic variation of Plasmodium knowlesi malaria: identification of the variant antigen on infected erythrocytes.

Erythrocytes infected with mature asexual stages of Plasmodium knowlesi express a new surface antigen such that rhesus monkey antisera specifically agglutinate these cells, and these antigens are malarial proteins rather than modified host proteins.

Isolate-specific S-antigen of Plasmodium falciparum contains a repeated sequence of eleven amino acids

Antibodies raised against a Plasmodium falciparum protein expressed in Escherichia coli reacted with a 220,000-molecular weight antigen of mature blood-stage parasites. The protein resembles the

Cloning and characterisation of the rRNA genes from the human malaria parasite Plasmodium falciparum.

Ribosomal DNA fragments from the human malaria parasite Plasmodium falciparum have been cloned and analysed in detail and copy number analysis reveals that each fragment is represented approximately 4 times in the genome, and implies that there are a total of 8 rRNA genes organised into at least two classes of transcription unit.

Protective immunization of the squirrel monkey against asexual blood stages of Plasmodium falciparum by use of parasite protein fractions.

Analysis of specific anti-malarial antibodies revealed a homogeneous response of all the animals against a few polypeptides of the mature parasite, indicating strong protection against challenge by the homologous strain P. falciparum.

Expression of strain‐specific surface antigens on Plasmodium falciparum‐infected erythrocytes

Antigenic determinants present on the surface of squirrel monkey erythrocytes infected with late developmental stages of P. falciparum were recognized through their ability to bind antibodies present

Protective antibodies against erythrocytic stages of Plasmodium falciparum in experimental infection of the squirrel monkey, Saimiri sciureus

The comparative immunochemical analysis of antigens recognized by protective and non‐protective antibodies revealed quantitative differences which may be of use for the identification of antIGens inducing protection.

Identification and chemical synthesis of a tandemly repeated immunogenic region of Plasmodium knowlesi circumsporozoite protein

Complementary DNA clones that code for the immunogenic region of the Plasmodium knowlesi circumsporozoite protein were shown to contain a tandemly repeating 36-base pair unit that accounts for at least one-third of the amino acid sequence of the surface antigen protein.