Human adult epidermal melanocytes cultured without chemical mitogens express the EGF receptor and respond to EGF

@article{Szabad2007HumanAE,
  title={Human adult epidermal melanocytes cultured without chemical mitogens express the EGF receptor and respond to EGF},
  author={G{\'a}bor Szabad and Bernadett Kormos and Andor Pivarcsi and M{\'a}rta Sz{\'e}ll and Korn{\'e}lia Kis and Anna Kenderessy Szab{\'o} and Attila Dobozy and Lajos Kem{\'e}ny and Zsuzsanna Bata-Cs{\"o}rgő},
  journal={Archives of Dermatological Research},
  year={2007},
  volume={299},
  pages={191-200}
}
We describe a novel chemical mitogen-free in vitro culture technique for obtaining pure melanocyte cultures using normal human adult epidermis as a source. The culture medium consists equal parts of the commercially available Keratinocyte Basal and AIM-V media (both from Gibco), as basal medium, which is supplemented with fetal bovine serum, bovine pituitary extract and recombinant human epidermal growth factor (EGF). Melanocytes harvested from human adult skin proliferate extensively and can… CONTINUE READING
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