Human Wharton’s Jelly Stem Cells Have Unique Transcriptome Profiles Compared to Human Embryonic Stem Cells and Other Mesenchymal Stem Cells

@article{Fong2010HumanWJ,
  title={Human Wharton’s Jelly Stem Cells Have Unique Transcriptome Profiles Compared to Human Embryonic Stem Cells and Other Mesenchymal Stem Cells},
  author={Chui-Yee Fong and Li-Ling Chak and Arijit Biswas and J Tan and Kalamegam Gauthaman and Woon Khiong Chan and Ariff Bongso},
  journal={Stem Cell Reviews and Reports},
  year={2010},
  volume={7},
  pages={1-16}
}
The human umbilical cord that originates from the embryo is an extra-embryonic membrane and the Wharton’s jelly within it is a rich source of stem cells (hWJSCs). It is not definitely known whether these cells behave as human embryonic stem cells (hESCs), human mesenchymal stem cells (hMSC) or both. They have the unique properties of high proliferation rates, wide multipotency, hypoimmunogenicity, do not induce teratomas and have anticancer properties. These advantages are important… Expand
Extra-embryonic human Wharton's jelly stem cells do not induce tumorigenesis, unlike human embryonic stem cells.
TLDR
Human WJSC, being hypoimmunogenic and non-tumorigenic, have the potential for safe cell-based therapies and are isolated and characterized from the extra-embryonic human umbilical cord Wharton's jelly. Expand
Common Expression of Stemness Molecular Markers and Early Cardiac Transcription Factors in Human Wharton's Jelly-Derived Mesenchymal Stem Cells and Embryonic Stem Cells
TLDR
This study demonstrated for the first time that WJ-MSCs had significant expression of undifferentiated human embryonic stem cell core markers, such as SOX2, NANOG, LIN28, S SEA1, SSEA3,SSEA4, KLF4, c-MYC, CRIPTO, and REX1, with a relatively lower level of expression than in hESCs. Expand
Single-cell RNA-seq highlights heterogeneity in human primary Wharton’s Jelly mesenchymal stem/stromal cells cultured in vitro
TLDR
Investigation of gene-expression heterogeneity of human primary Wharton’s Jelly-derived MSCs cultured in vitro via single-cell RNA-seq suggested that these HVGs hold the potential to be used as candidate markers for further potency association studies. Expand
Single-cell RNA-seq highlights heterogeneity in human primary Wharton’s jelly mesenchymal stem/stromal cells cultured in vitro
TLDR
HVGs identified in MSCs are associated with functional characteristics, on which the MSC clinical application were theoretically based, such as development and inflammation response, and hold the potential to be used as candidate markers for further potency association studies. Expand
Differentiation of the definitive endoderm from Wharton ’ s Jelly Mesenchymal Stem Cells ( WJMSC )
The umbilical cord is a rich source of stem cells with high proliferation and differentiation capabilities (Weiss & Troyer, 2006). Wharton’s jelly is the conne cti ve tissue of the umbilical cordExpand
Human Wharton's Jelly-Derived Stem Cells Display a Distinct Immunomodulatory and Proregenerative Transcriptional Signature Compared to Bone Marrow-Derived Stem Cells.
TLDR
Gene ontology analysis revealed that genes associated with cell adhesion, proliferation, and immune system functioning are enriched in WJ-MSC, which makes it an attractive candidate for cell-based therapy in neurodegenerative and immune-mediated central nervous system disorders. Expand
Immune characterization of mesenchymal stem cells in human umbilical cord Wharton's jelly and derived cartilage cells.
TLDR
Their intermediate state between adult and embryonic stem cells makes them an ideal candidate for reprogramming to the pluripotent status and additional studies are necessary to clarify the potential of hWJMSCs to be used in cartilage and other tissue regeneration and cell-based therapies. Expand
An in vitro model for hepatocyte-like cell differentiation from Wharton’s jelly derived-mesenchymal stem cells by cell-base aggregates
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A protocol for UCMSC differentiation into hepatocytes was established and these cells were morphologically and functionally similar to hepatocytes, suggesting hepatocyte differentiation may be facilitated by the UCMSCs aggregate formation before administration of the differentiation protocols. Expand
Molecular and Functional Verification of Wharton’s Jelly Mesenchymal Stem Cells (WJ-MSCs) Pluripotency
TLDR
WJ-MSCs express some pluripotency markers—such as NANOG, OCT-4, and SSEA-4—but in comparison to iPS cells expression level is significantly lower, and the level of expression can be raised under hypoxic conditions. Expand
Mesenchymal stem cell separation from Wharton’s jelly and its differentiation into keratinocytes
TLDR
Findings in this work may have a significant impact on studies of early human epithelial differentiation, functional genomics, pharmacological testing, cell therapy, and tissue engineering by helping to eliminate worrying ethical and technical issues. Expand
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