Human SUMO fusion systems enhance protein expression and solubility.

  title={Human SUMO fusion systems enhance protein expression and solubility.},
  author={Zhongyuan Wang and H Guiyun Li and Wei Guan and Haili Ling and Zhiyong Wang and Tianyang Mu and Franklin D. Shuler and Xuexun Fang},
  journal={Protein expression and purification},
  volume={73 2},
A major challenge associated with recombinant protein production in Escherichia coli is generation of large quantities of soluble, functional protein. Yeast SUMO (small ubiquitin-related modifier), has been shown to enhance heterologous protein expression and solubility as fusion tag, however, the effects of human SUMOs on protein expression have not been investigated. Here we describe the use of human SUMO1 and SUMO2 as a useful gene fusion technology. Human SUMO1 and SUMO2 fusion expression… CONTINUE READING


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