Human Rhinovirus 87 Identified as Human Enterovirus 68 by VP4-Based Molecular Diagnosis

  title={Human Rhinovirus 87 Identified as Human Enterovirus 68 by VP4-Based Molecular Diagnosis},
  author={H. Ishiko and R. Miura and Y. Shimada and A. Hayashi and H. Nakajima and S. Yamazaki and N. Takeda},
  pages={136 - 141}
  • H. Ishiko, R. Miura, +4 authors N. Takeda
  • Published 2002
  • Biology, Medicine
  • Intervirology
  • Human rhinoviruses (HRVs) are the major cause of respiratory infections. We developed a diagnostic method for HRVs based on the reverse-transcription polymerase chain reaction (RT-PCR) and VP4-based phylogenetic analysis. A set of primers used in the RT-PCR of human enteroviruses (EVs) appeared to be capable of amplifying all prototype strains of HRVs, each of which generated a 530-bp fragment. The single exception was HRV-87, which generated a 650-bp fragment, as observed in human EVs. The VP4… CONTINUE READING
    60 Citations
    Molecular characterization of human rhinovirus field strains isolated during surveillance of enteroviruses.
    • 21
    Rhinovirus detection using different PCR-based strategies
    • 2
    • PDF
    Amplicon Sequencing and Improved Detection of Human Rhinovirus in Respiratory Samples
    • 70
    • PDF
    VP1 Sequencing of All Human Rhinovirus Serotypes:Insights into Genus Phylogeny and Susceptibility to AntiviralCapsid-BindingCompounds
    • 180
    • PDF
    Enterovirus 68 is associated with respiratory illness and shares biological features with both the enteroviruses and the rhinoviruses.
    • 219
    Newly identified human rhinoviruses: molecular methods heat up the cold viruses
    • 77
    Human rhinovirus C associated with wheezing in hospitalised children in the Middle East.
    • E. Miller, N. Khuri-bulos, +9 authors N. Halasa
    • Biology, Medicine
    • Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
    • 2009
    • 117
    Species-specific RT-PCR amplification of human enteroviruses: a tool for rapid species identification of uncharacterized enteroviruses.
    • 171
    Upsurge of human enterovirus 68 infections in patients with severe respiratory tract infections.
    • 106


    Molecular diagnosis of human enteroviruses by phylogeny-based classification by use of the VP4 sequence.
    • 133
    • PDF
    Detection and differentiation of picornaviruses in clinical samples following genomic amplification.
    • 148
    Molecular evolution of the major capsid protein VP1 of enterovirus 70.
    • 73
    • PDF
    Nucleic acid detection systems for enteroviruses.
    • H. Rotbart
    • Biology, Medicine
    • Clinical Microbiology Reviews
    • 1991
    • 71
    • PDF
    Complete nucleotide sequence of the attenuated poliovirus Sabin 1 strain genome.
    • A. Nomoto, T. Omata, +6 authors N. Imura
    • Biology, Medicine
    • Proceedings of the National Academy of Sciences of the United States of America
    • 1982
    • 240
    • PDF
    Enteroviral infections of the central nervous system.
    • H. Rotbart
    • Medicine
    • Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
    • 1995
    • 283
    • J. Felsenstein
    • Medicine, Biology
    • Evolution; international journal of organic evolution
    • 1985
    • 36,171
    • PDF