Human Rhinovirus 87 Identified as Human Enterovirus 68 by VP4-Based Molecular Diagnosis

@article{Ishiko2002HumanR8,
  title={Human Rhinovirus 87 Identified as Human Enterovirus 68 by VP4-Based Molecular Diagnosis},
  author={Hiroaki Ishiko and Rika Miura and Yasushi Shimada and Akio Hayashi and Haruhiko Nakajima and Shudo Yamazaki and Naokazu Takeda},
  journal={Intervirology},
  year={2002},
  volume={45},
  pages={136 - 141}
}
Human rhinoviruses (HRVs) are the major cause of respiratory infections. We developed a diagnostic method for HRVs based on the reverse-transcription polymerase chain reaction (RT-PCR) and VP4-based phylogenetic analysis. A set of primers used in the RT-PCR of human enteroviruses (EVs) appeared to be capable of amplifying all prototype strains of HRVs, each of which generated a 530-bp fragment. The single exception was HRV-87, which generated a 650-bp fragment, as observed in human EVs. The VP4… 
Molecular characterization of human rhinovirus field strains isolated during surveillance of enteroviruses.
TLDR
Analysis of amino acids corresponding to the intercellular adhesion molecule-1 receptor footprint in major receptor group HRVs and also in the low-density lipoprotein receptor footprint of minor receptorGroup HRVs showed conservation of the 'minor receptor group-like' amino acids, indicating that the field strains may have maintained their minor receptor group specificity.
Rhinovirus detection using different PCR-based strategies
TLDR
The semi-nested protocol utilized in the present study was less sensitive and was not useful in differentiating HRV from HEV, the major cause of the common cold.
Amplicon Sequencing and Improved Detection of Human Rhinovirus in Respiratory Samples
TLDR
It is shown that to specifically identify HRV in clinical specimens, diagnostic assays need to overcome both the diversities and the similarities of picornaviruses.
VP1 Sequencing of All Human Rhinovirus Serotypes:Insights into Genus Phylogeny and Susceptibility to AntiviralCapsid-BindingCompounds
TLDR
Observations suggest that it is possible to differentiate rhinoviruses naturally resistant to capsid function inhibitors from those that emerge from susceptible virus populations as a result of antiviral drug selection pressure based on sequence analysis of the drug-binding pocket.
Enterovirus 68 is associated with respiratory illness and shares biological features with both the enteroviruses and the rhinoviruses.
TLDR
It is concluded that EV68 is primarily an agent of respiratory disease and that it shares important biological and molecular properties with both the enteroviruses and the rhinoviraluses.
Newly identified human rhinoviruses: molecular methods heat up the cold viruses
TLDR
The findings that trigger the questions arising during the current cycle of intense rhinovirus discovery are reviewed, including whether clinical HRV detections are best described as a distinct strain or a closely related variant of a previously identified strain (or serotype).
Molecular epidemiology of human rhinoviruses
TLDR
This study is the first systematic genetic characterization of all known HRV prototype strains, providing a further taxonomic proposal for classification of HRV, and proposed to divide the genus Human rhinoviruses into HRV-A andHRV-B.
Human rhinovirus C associated with wheezing in hospitalised children in the Middle East.
  • E. Miller, N. Khuri-Bulos, +9 authors N. Halasa
  • Biology, Medicine
    Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
  • 2009
TLDR
There is a significant burden of HRV-associated hospitalisations in young children in Jordan and infection with the recently identified group HRVC is associated with wheezing and more severe illness.
Enterovirus D68: molecular biological characteristics, features of infection
Human enteroviruses (genus Enterovirus, family Picornaviridae) are infectious agents characterized by a wide range of clinical manifestations. EV-D68, associated with respiratory and neurological
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 12 REFERENCES
Molecular diagnosis of human enteroviruses by phylogeny-based classification by use of the VP4 sequence.
TLDR
This study investigated a rapid and reliable method based on reverse-transcription polymerase chain reaction and phylogenetic analysis that appears to be an effective tool for the molecular epidemiology study of EVs.
Detection and differentiation of picornaviruses in clinical samples following genomic amplification.
TLDR
The PCR definitively identified poliovirus and CVs from the CSF or stool of patients with aseptic meningitis, as well as CV in the pericardial fluid of a patient who had suffered a myocardial infarction.
Genetic clustering of all 102 human rhinovirus prototype strains: serotype 87 is close to human enterovirus 70.
TLDR
The genetic relationships of HRV prototype strains and the possibility of using genetic identification of a given HRV field strain were studied, and larger numbers of field isolates of known serotype need to be characterized, possibly also in the VP1 region, to evaluate the feasibility of genetic typing of HRVs.
Molecular Evolution of the Human Enteroviruses: Correlation of Serotype with VP1 Sequence and Application to Picornavirus Classification
TLDR
The data suggest that VP1 sequence comparisons may be valuable in enterovirus typing and in picornavirus taxonomy by assisting in the genus assignment of unclassified picsornaviruses.
Molecular evolution of the major capsid protein VP1 of enterovirus 70
TLDR
The phylogenetic tree indicated that the virus had evolved from one focal place, and the time of emergence was estimated to be August 1967 +/- 15 months, 2 years before first recognition of the pandemic of acute hemorrhagic conjunctivitis.
Nucleic acid detection systems for enteroviruses
  • H. Rotbart
  • Medicine, Biology
    Clinical Microbiology Reviews
  • 1991
TLDR
The enteroviruses comprise nearly 70 human pathogens responsible for a wide array of diseases including poliomyelitis, meningitis, myocarditis, and neonatal sepsis and similar approaches to the study of enteroviral pathogenesis have produced dramatic advances in understanding of how these important viruses cause their diverse clinical spectra.
Complete nucleotide sequence of the attenuated poliovirus Sabin 1 strain genome.
  • A. Nomoto, T. Omata, +6 authors N. Imura
  • Biology, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 1982
TLDR
A cluster of amino acid changes is located in the viral coat proteins, especially in the NH2-terminal half of the viral capsid protein VP1, which may imply that the mutations in the VP1 coding region contribute to attenuation.
Comparative sequence analysis of the 5' noncoding region of the enteroviruses and rhinoviruses.
TLDR
A comparative sequence analysis of the 5' noncoding region of a subgroup of the picornavirus, including the polioviruses, coxsackie B3, and the human rhinovirus, reveals the conservation of certain features despite the divergence of sequence, implicates a biologically functional role for this region.
Enteroviral infections of the central nervous system.
  • H. Rotbart
  • Medicine
    Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
  • 1995
TLDR
The clinical presentation may mimic that of bacterial or other viral CNS infections, a circumstance making laboratory diagnosis of paramount importance for reducing unnecessary hospitalization and therapy.
CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAP
  • J. Felsenstein
  • Biology, Medicine
    Evolution; international journal of organic evolution
  • 1985
TLDR
The recently‐developed statistical method known as the “bootstrap” can be used to place confidence intervals on phylogenies and shows significant evidence for a group if it is defined by three or more characters.
...
1
2
...