Human PKR transfected into murine cells stimulates expression of genes under control of the HIV1 or HTLV-I LTR.

@article{Meurs1995HumanPT,
  title={Human PKR transfected into murine cells stimulates expression of genes under control of the HIV1 or HTLV-I LTR.},
  author={Eliane F Meurs and Nigel A. J. McMillan and Bret R. Williams and Ara G. Hovanessian and Peter J. Southern},
  journal={Virology},
  year={1995},
  volume={214 2},
  pages={653-9}
}
We have analyzed the effect of transfection into murine NIH/3T3 cells of the human dsRNA-activated kinase PKR on the expression of the beta-galactosidase reporter gene, placed under control of the HIV1 or the HTLV-I LTR. beta-Galactosidase expression is stimulated when the reporter plasmids are cotransfected with wild-type PKR but inhibited when cotransfected with a catalytically inactive mutant PKR. In the case of HIV1, beta-galactosidase expression was not stimulated when cotransfection was… CONTINUE READING