Human Macrophages Accumulate HIV‐1 Particles in MHC II Compartments

@article{Raposo2002HumanMA,
  title={Human Macrophages Accumulate HIV‐1 Particles in MHC II Compartments},
  author={Graça Raposo and Margaret Moore and Donald Innes and Richtje Leijendekker and Andrew Leigh-Brown and Philippe Benaroch and Hans J. Geuze},
  journal={Traffic},
  year={2002},
  volume={3}
}
Macrophages are important targets for HIV‐1 infection and harbor the virions in an as yet unidentified organelle. To determine the location of HIV‐1 in these cells, an extensive analysis of primary human macrophages infected in vitro with HIV‐1 was carried out by immuno‐electron microscopy. Virus particles were found to accumulate in intracellular multivesicular compartments which were enriched in major histocompatibility complex class II molecules and CD63. These features are characteristics… 
Major Histocompatibility Complex Class II Molecules Promote Human Immunodeficiency Virus Type 1 Assembly and Budding to Late Endosomal/Multivesicular Body Compartments
TLDR
A role of MHC-II molecules in promoting HIV-1 assembly and budding to LE/MVB is suggested and the possibility that this activity might be part of a normal pathway of virus production in cell types physiologically expressing MHC -II molecules, such as macrophages is raised.
Infectious HIV-1 assembles in late endosomes in primary macrophages
TLDR
The data indicate that most of the infectious HIV produced by primary macrophages is assembled on late endocytic membranes and acquires antigens characteristic of this compartment, which has significant implications for understanding the biology of HIV and its cell–cell transmission.
HIV-1 Buds Predominantly at the Plasma Membrane of Primary Human Macrophages
TLDR
The composition of cellular marker proteins incorporated into HIV-1 supported a plasma membrane–derived origin of the viral envelope, contrary to current opinion, the plasma membrane is the primary site of HIV- 1 budding also in infected macrophages.
HIV-1 assembly in macrophages
TLDR
The complete process of HIV-1 assembly is still poorly understood and will undoubtedly benefit from the ongoing explosion of new imaging techniques allowing better time-lapse and quantitative studies.
Reviewassembly in macrophages
TLDR
The complete process of HIV-1 assembly is still poorly understood and will undoubtedly benefit from the ongoing explosion of new imaging techniques allowing better time-lapse and quantitative studies.
CD63 Is Not Required for Production of Infectious Human Immunodeficiency Virus Type 1 in Human Macrophages
TLDR
It is shown that in the absence of CD63, HIV assembly is quantitatively comparable to that seen in CD63-expressing macrophages and that virus assembly occurs on compartments positive for CD81, CD9, and CD53.
Assembly of infectious HIV-1 in human epithelial and T-lymphoblastic cell lines.
Macrophages and HIV-1: dangerous liaisons.
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