Human Luteinizing hormone-releasing hormone gene (LHRH) is located on short arm of chromosome 8 (region 8p11.2 → p21)

  title={Human Luteinizing hormone-releasing hormone gene (LHRH) is located on short arm of chromosome 8 (region 8p11.2 → p21)},
  author={Teresa L. Yang-Feng and P. H. Seeburg and Uta Francke},
  journal={Somatic Cell and Molecular Genetics},
Luteinizing hormone-releasing hormone (LHRH) is synthesized by hypothalamic neurons and affects the release of gonadotropic hormones from the anterior pituitary gland. A cDNA clone encoding the human LHRH precursor molecule was used to assign theLHRH gene to a human chromosome by in situ hybridization and Southern blot analysis. Metaphase spreads from two normal individuals were hybridized with3H -labeled LHRH-specific sequence. Of 120 cells analyzed, 33 had silver grains over the p11.2 → p21… 
The gonadotropin-releasing hormone (Gnrh) gene maps to mouse chromosome 14 and identifies a homologous region on human chromosome 8
The localization of Gnrh, the murine homolog of luteinizing hormone-releasing hormone, to mouse chromosome 14 defines a hitherto unrecognized block of homology between man and mouse, and indicates that the region ofhomology between the human chromosome 8 short arm and mouse chromosome 8 is composed of two separate blocks.
Expression of mRNA for human type-I LHRH receptor transcript forms in human benign prostatic hyperplasia.
The results suggest that L HRH-R-I gene may have more than two splice variants or uncharacterised transcript forms of LHRH- R-I, and support the merit of further investigation of the expression of this gene and its transcript forms in human BPH.
Subregional mapping of the human gonadotropin-releasing hormone receptor (GnRH-R) gene to 4q between the markers D4S392 and D4S409
Nine yeast artificial chromosomes containing the gene that encodes the human gonadotropin-releasing hormone receptor (GnRH-R) gene are isolated by screening the YAC library of the Centre d'Etude du Polymorphisme Humain by the use of the polymerase chain reaction.
Isolation and characterization of the human gonadotropin-releasing hormone gene in the hypothalamus and placenta.
The GnRH gene has been cloned in several species, but the location of the promoter and the exact start of transcription have not previously been determined, and this characterization will allow hormonal regulatory studies to be performed using gene transfer techniques.
Second gene for gonadotropin-releasing hormone in humans.
Molecular phylogenetic analysis shows that this second GnRH-II gene is likely the result of a duplication before the appearance of vertebrates, and predicts the existence of a third GnRH form in humans and other vertebrates.
Processing of the luteinizing hormone-releasing hormone precursor in the preoptic area and hypothalamus of the rat.
It is demonstrated that multiple IR forms of the LHRH prohormone exist in the POH of the rat and that nerve terminals of theLHRH neurons contain L HRH, GAP1-56, and some lower mol wt GAP-like substances.
Molecular biology of gonadotropin-releasing hormone (GnRH)-I, GnRH-II, and their receptors in humans.
Functionally, there is growing evidence showing that both GnRH-I and Gn RH-II are potentially important autocrine and/or paracrine regulators in some extrapituitary compartments.
Normal structure of the gonadotropin-releasing hormone (GnRH) gene in patients with GnRH deficiency and idiopathic hypogonadotropic hypogonadism.
It is concluded that a major rearrangement of the GnRH gene is not a common basis for IHH in humans, and polymorphisms or deletions or rearrangements involving more than 200 basepairs were excluded.


Characterization of cDNA for precursor of human luteinizing hormone releasing hormone
Cloned genomic and cDNA sequences encoding the precursor form of LHRH, the existence of which had been suggested from Chromatographic studies of hypothalamic8,13 and placental8 extracts, are isolated.
Intrachromosomal gene mapping in man: Assignment of nucleoside phosphorylase to region 14cen→14q21 by interspecific hybridization of cells with a t(X;14) (p22;q21) translocation
Results indicate localization of the NP gene in region 14pter→14q21, which has been assigned to a subregion of chromosome 14 by somatic cell hybridization of male and female cells containing the balanced translocation t(X;14) (p22;q21).
Assignment of the major histocompatibility complex to a region of the short arm of human chromosome 6.
  • U. Francke, M. Pellegrino
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1977
Quantitative correlation between the density of HLA antigens on the hybrid cell surface and the number of copies of the respective HLA gene-bearing chromosome suggests a gene dose effect for cell surface molecules, as it exists for intracellular gene products.
A prolactin-inhibiting factor within the precursor for human gonadotropin-releasing hormone
The cloned complementary DNA sequence encoding the human gonadotropin-releasing hormone (GnRH) precursor protein was used to construct an expression vector for the bacterial synthesis of the 56-amino
Assignment of first random restriction fragment length polymorphism (RFLP) locus ((D14S1) to a region of human chromosome 14.
A locus responsible for a restriction fragment length polymorphism (RFLP) has been identified by hybridization of Eco RI fragments to the random human DNA sequence in recombinant plasmid pAW101 by assigning the first highly polymorphic random RFPL locus (D14S1) to region q21 leads to qter of chromosome 14.
Random X inactivation resulting in mosaic nullisomy of region Xp21.1----p21.3 associated with heterozygosity for ornithine transcarbamylase deficiency and for chronic granulomatous disease.
  • U. Francke
  • Biology, Medicine
    Cytogenetics and cell genetics
  • 1984
The cells from this patient and the somatic cell hybrids containing her deleted X chromosome in the absence of the normal X provide material for the precise mapping of X linked genes and DNA sequences on the short arm of the human X chromosome.
Gonadotrophin-releasing hormone deficiency in a mutant mouse with hypogonadism
The genetic and endocrinological background of a mutant strain of mouse in which the testes and ovaries fail to develop postnatally is reported, which should prove useful for studying the synthesis of hypothalamic releasing hormones as well as the role of the hypothalamic–gonadotrophin system in sexual differentiation, puberty, folliculogenesis and spermatogenesis.
Gonadocrinins: Peptides in Ovarian Follicular Fluid Stimulating the Secretion of Pituitary Gonadotropins*
A newly discovered small peptide purified from rat follicular fluid stimulates the pituitary to release FSH and LH in vitro as well as in vivo, and is named gonadocrinin for operational facility.
Brain grafts reverse hypogonadism of gonadotropin releasing hormone deficiency
Fetal preoptic area (POA) from unaffected animals of the hpg strain was transplanted into the anterior third ventricle of adult hpg mice and Hypothalamic GnRH and pituitary and plasma gonadotropin concentrations were increased compared with levels in untreated animals.
Partial isolation and characterization of testicular GnRH-like factors.
It is concluded that there are at least two factors in rat testis with GnRH-receptor-binding properties that are chemically distinct from the native decapeptide.