Human-Induced Pluripotent Stem Cells: In Quest of Clinical Applications

  title={Human-Induced Pluripotent Stem Cells: In Quest of Clinical Applications},
  author={Rosalinda Madonna},
  journal={Molecular Biotechnology},
  • R. Madonna
  • Published 3 February 2012
  • Biology
  • Molecular Biotechnology
In the field of regenerative medicine, the development of induced pluripotent stem (iPS) cells may represent a potential strategy to overcome the limitations of human embryonic stem cells (ESCs). iPS cells have the potential to mimic human disease, since they carry the genome of the donor. Hypothetically, with iPS cell technology it is possible to screen patients for a genetic cause of disease (genetic mutation), develop cell lines, reprogram them back to iPS cells, finally differentiate them… 

Impact of New Genome Editing Tools on iPS Cell Based Therapies

With the recent advancement of new gene editing tools which allow site specific DNA targeting, HR efficiency in iPS cells have been significantly improved and among these new tools, ZFNs, TALENs and CRISPRs are the most promising ones.

Application of iPS Cell Technology for Cardiac Disease Modeling and Repair

Since the advent of induced pluripotent stem (iPS) cell technology by Yamanaka and colleagues in 2006, demonstrating that mouse embryonic fibroblasts can be reprogrammed to an embryonic–like state by

Challenges and possible clinical applications of human embryonic stem cell research

Human embryonic stem cells (hESC) offer a unique insight into basic human development in vitro, allow better understanding of the genetic and molecular controls of these processes, and are of pharmaceutical interest to test or develop new drugs.

Comparison of different protocols for neural differentiation of human induced pluripotent stem cells

Neural differentiation potential of three induction media for conversion of hiPSCs into neural lineages is compared and results indicated a successful establishment protocol with high efficiency for differentiation of hi PSCs into Neural lineages.

From pluripotency to distinct cardiomyocyte subtypes.

Recent progress in the cardiovascular stem cell field is discussed addressing the availability of specific cardiac cellular subtypes and the recent achievement of directly reprogramming fibroblasts into cardiomyocytes via a milder approach may become of great importance.

Characterization of common marmoset dysgerminoma-like tumor induced by the lentiviral expression of reprogramming factors

The results indicate that DNA‐damaging agents, irradiation, fibroblast growth factor receptor inhibitor, and c‐MYC‐targeted therapies might represent effective treatment strategies for unexpected tumors in patients receiving iPSC‐based therapy.

Endometrial Stem Cells as Potential Cures for Human Diseases

Preliminary data show in preliminary data that human endometrium contains multipotent mesenchymal stem cells that can be differentiated into chondrocytes, adipocytes, myocytes, neurons, and pancreatic beta-like cells that secrete insulin.

Introduction to Stem Cell Principles and Biology

  • M. Roubelakis
  • Biology
    Stem Cell Biology and Regenerative Medicine
  • 2019
The type of human cells, the use of growth factors and cytokines to stimulate the production, and growth and function of cells, along with the cell sources, have shown a significant therapeutic impact to date and represent a rapidly grown area of regenerative medicine.



Generation of mouse-induced pluripotent stem cells with plasmid vectors

An alternative method to generate iPS cells from mouse embryonic fibroblasts (MEFs) by continual transfection of plasmid vectors is described, which reduces the safety concern for iPS cell generation and application, and provides a source of cells for the investigation of the mechanisms underlying reprogramming and pluripotency.

Immunogenicity of induced pluripotent stem cells.

A new study shows the immunogenicity of induced pluripotent stem cells by the direct transplantation of undifferentiated cells into syngenic mice, indicating that cells differentiated from iPSCs can at least partially replace the biological functions of various organs.

Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells

Somatic cell nuclear transfer allows trans-acting factors present in the mammalian oocyte to reprogram somatic cell nuclei to an undifferentiated state. We show that four factors (OCT4, SOX2, NANOG,

Generation of Induced Pluripotent Stem Cell Lines from Tibetan Miniature Pig*

The successful generation of pluripotent stem cells from fibroblasts isolated from the Tibetan miniature pig using a modified iPS protocol more closely resemble human ESC than cells from other species is reported.

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It is shown that corrected Fanconi-anaemia-specific iPS cells can give rise to haematopoietic progenitors of the myeloid and erythroid lineages that are phenotypically normal, that is, disease-free.

Reprogramming of murine and human somatic cells using a single polycistronic vector

A novel approach to reduce the number of viruses necessary to reprogram somatic cells by delivering reprogramming factors in a single virus using 2A “self-cleaving” peptides, which support efficient polycistronic expression from a single promoter.

Induced Pluripotent Stem Cells Generated from Patients with ALS Can Be Differentiated into Motor Neurons

Induced pluripotent stem cells are generated from an 82-year-old woman diagnosed with a familial form of amyotrophic lateral sclerosis and were successfully directed to differentiate into motor neurons, the cell type destroyed in ALS.