Human IgA-binding peptides selected from random peptide libraries: affinity maturation and application in IgA purification.

@article{Hatanaka2012HumanIP,
  title={Human IgA-binding peptides selected from random peptide libraries: affinity maturation and application in IgA purification.},
  author={Takaaki Hatanaka and Shinji Ohzono and Mirae Park and Kotaro Sakamoto and Shogo Tsukamoto and Ryohei Sugita and Hiroyuki Ishitobi and Toshiyuki Mori and Osamu Ito and Koichi Sorajo and Kazuhisa Sugimura and Sihyun Ham and Yuji Ito},
  journal={The Journal of biological chemistry},
  year={2012},
  volume={287 51},
  pages={43126-36}
}
Phage display system is a powerful tool to design specific ligands for target molecules. Here, we used disulfide-constrained random peptide libraries constructed with the T7 phage display system to isolate peptides specific to human IgA. The binding clones (A1-A4) isolated by biopanning exhibited clear specificity to human IgA, but the synthetic peptide… CONTINUE READING