Human DNA ligase I completely encircles and partially unwinds nicked DNA

@article{Pascal2004HumanDL,
  title={Human DNA ligase I completely encircles and partially unwinds nicked DNA},
  author={John M. Pascal and Patrick J. O'Brien and Alan E Tomkinson and Tom E. Ellenberger},
  journal={Nature},
  year={2004},
  volume={432},
  pages={473-478}
}
The end-joining reaction catalysed by DNA ligases is required by all organisms and serves as the ultimate step of DNA replication, repair and recombination processes. One of three well characterized mammalian DNA ligases, DNA ligase I, joins Okazaki fragments during DNA replication. Here we report the crystal structure of human DNA ligase I (residues 233 to 919) in complex with a nicked, 5′ adenylated DNA intermediate. The structure shows that the enzyme redirects the path of the double helix… Expand
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  • Biology, Medicine
  • The Journal of Biological Chemistry
  • 2009
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