Human Cytidine Deaminase: Understanding the Catalytic Mechanism

  title={Human Cytidine Deaminase: Understanding the Catalytic Mechanism},
  author={Stefano Costanzi and Silvia Vincenzetti and Alberto Vita and Catia Lambertucci and Sara Taffi and Rosaria Volpini and Sauro Vittori and Gloria Cristalli},
  journal={Nucleosides, Nucleotides \& Nucleic Acids},
  pages={1539 - 1543}
Abstract In the absence of an experimentally elucidated three-dimensional structure of the human CDA, we built an homology model of this enzyme starting from the crystal structure of its E. coli homologous. Furthermore, we docked in the active site alternatively the substrate, the intermediate or the product. By means of molecular dynamics simulations, we determined the topology of the active site, identifying the amino acids involved in the catalytic mechanism, and outlining the central role… 
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Figure 2. Human CDA catalytic mechanism