Human CUL1 forms an evolutionarily conserved ubiquitin ligase complex (SCF) with SKP1 and an F-box protein.

@article{Lyapina1998HumanCF,
  title={Human CUL1 forms an evolutionarily conserved ubiquitin ligase complex (SCF) with SKP1 and an F-box protein.},
  author={Svetlana Lyapina and Carl C. Correll and Edward T Kipreos and Raymond J Deshaies},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1998},
  volume={95 13},
  pages={7451-6}
}
The SCF ubiquitin ligase complex of budding yeast triggers DNA replication by catalyzing ubiquitination of the S phase cyclin-dependent kinase inhibitor SIC1. SCF is composed of three proteins-ySKP1, CDC53 (Cullin), and the F-box protein CDC4-that are conserved from yeast to humans. As part of an effort to identify components and substrates of a putative human SCF complex, we isolated hSKP1 in a two-hybrid screen with hCUL1, the closest human homologue of CDC53. Here, we show that hCUL1… CONTINUE READING

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