Human Antibodies with Sub-nanomolar Affinities Isolated from a Large Non-immunized Phage Display Library

@article{Vaughan1996HumanAW,
  title={Human Antibodies with Sub-nanomolar Affinities Isolated from a Large Non-immunized Phage Display Library},
  author={Tristan J. Vaughan and Andrew James Williams and Kevin Pritchard and Jane K. Osbourn and Anthony Richard Pope and John C. Earnshaw and John McCafferty and Regina A. Hodits and Jane Wilton and Kevin Stuart Johnson},
  journal={Nature Biotechnology},
  year={1996},
  volume={14},
  pages={309-314}
}
To generate a stable resource from which high affinity human antibodies to any given antigen can be rapidly isolated, functional V-gene segments from 43 non-immunized human donors were used to construct a repertoire of 1.4 × 1010 single-chain Fv (scFv) fragments displayed on the surface of phage. Fragments were cloned in a phagemid vector, enabling both phage displayed and soluble scFv to be produced without subcloning. A hexahistidine tag has been incorporated to allow rapid purification of… Expand
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